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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3) (Internal Region) antibody
|Synonyms||PSAP2, PROSAP2, SPANK-2, KIAA1650, DEL22q13.3, Prosap2|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||SHANK3 (ProSAP2)|
|Immunogen||A synthetic peptide from the internal part of human SHANK3 (SH3 and multiple ankyrin repeat domains protein 3, ProSAP2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 97% identity with rat and mouse sequence.|
|Description||Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Subcellular location: Cytoplasm. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD. Tissue specificity: Widely expressed in brain. Also known as: SH3 and multiple ankyrin repeat domains protein 3, ProSAP2, Proline rich synapse associated protein 2, PSAP2.|
|Specificity||Appears to be specific for SHANK3.|
|Application Notes||IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).
Hirosawa, Nagase, Murahashi et al.: "Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 8, Issue 1, pp. 1-9, 2001 (PubMed).
Soltau, Richter, Kreienkamp: "The insulin receptor substrate IRSp53 links postsynaptic shank1 to the small G-protein cdc42." in: Molecular and cellular neurosciences, Vol. 21, Issue 4, pp. 575-83, 2002 (PubMed).
Durand, Betancur, Boeckers et al.: "Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are associated with autism spectrum disorders." in: Nature genetics, Vol. 39, Issue 1, pp. 25-7, 2006 (PubMed).
Zahedi, Lewandrowski, Wiesner et al.: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).
|Hosts||Rabbit (25), Mouse (7), Goat (1)|
|Reactivities||Human (26), Mouse (Murine) (24), Rat (Rattus) (23)|
|Applications||Immunofluorescence (IF) (18), Western Blotting (WB) (18), Immunohistochemistry (IHC) (10), ELISA (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunostaining (ISt) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||AA 840-857 (3), Internal Region (3), Middle Region (1), PDZ Domain (1), SH3 Domain (1)|