SHANK3 (SH3 and multiple ankyrin repeat domains protein 3, ProSAP2) (Internal Region) antibody

Antigen: SHANK3 (SH3 and multiple ankyrin repeat domains protein 3, ProSAP2)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350832

Additional Information

UniProt: Q9BYB0

Immunogen: A synthetic peptide from the internal part of human SHANK3 (SH3 and multiple ankyrin repeat domains protein 3, ProSAP2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 97% identity with rat and mouse sequence.

Format: Lyophilized

Isotype: IgG

Description: Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Subcellular location: Cytoplasm. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD. Tissue specificity: Widely expressed in brain. Also known as: SH3 and multiple ankyrin repeat domains protein 3, ProSAP2, Proline rich synapse associated protein 2, PSAP2.

Specificity: Appears to be specific for SHANK3.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Hirosawa, Nagase, Murahashi et al.: "Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 8, Issue 1, pp. 1-9, 2001 (PubMed).

Soltau, Richter, Kreienkamp: "The insulin receptor substrate IRSp53 links postsynaptic shank1 to the small G-protein cdc42." in: Molecular and cellular neurosciences, Vol. 21, Issue 4, pp. 575-83, 2002 (PubMed).

Durand, Betancur, Boeckers et al.: "Mutations in the gene encoding the synaptic scaffolding protein SHANK3 are associated with autism spectrum disorders." in: Nature genetics, Vol. 39, Issue 1, pp. 25-7, 2006 (PubMed).

Zahedi, Lewandrowski, Wiesner et al.: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).