SRC Kinase Signaling Inhibitor 1 (SRCIN1) (N-Term) antibody

Antigen: SRC Kinase Signaling Inhibitor 1 (SRCIN1)

Synonyms: SNIP, KIAA1684

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350837

Additional Information

Alternative name: SNIP (SNAP 25 interacting protein) (n-terminal)

Swiss-Prot: Q9C0H9

Immunogen: A synthetic peptide from the n-terminal of human SNIP (SNAP 25 interacting protein) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Delays the onset of cell spreading in the early stages of cell adhesion to fibronectin. Also involved in calcium-dependent exocytosis from PC12 cells. SUBUNIT: Interacts with the N-terminal coiled-coil region ofSNAP25. Interacts with BCAR1/p130Cas through its C-terminal domain. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note: Localized to the perinuclear region, lamellopodia, cortical actin and actin stress fibers but not to focal adhesions. Also known as: p130 Cas associated protein, p140 Cap,SNAP 25 interacting protein, P140.

Specificity: Appears to be specific for SNIP.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to n-terminal of SNIP (SNAP 25 interacting protein): whole serum (ABIN350837) at 1: 2000 dilution. DAPI counter stained.

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to n-terminal of SNIP (SNAP 25 interacting protein): whole serum (ABIN350837) at 1: 2000 dilution. DAPI counter stained appearing in blue.

Publications

Publications: Nagase, Kikuno, Hattori et al.: "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 6, pp. 347-55, 2001 (PubMed).

Di Stefano, Cabodi, Boeri Erba et al.: "P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading." in: Molecular biology of the cell, Vol. 15, Issue 2, pp. 787-800, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

DeGiorgis, Jaffe, Moreira et al.: "Phosphoproteomic analysis of synaptosomes from human cerebral cortex." in: Journal of proteome research, Vol. 4, Issue 2, pp. 306-15, 2005 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).