SRC Kinase Signaling Inhibitor 1 (SRCIN1) (N-Term) antibody

Details for Product No. ABIN350837
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Antigen
Synonyms P140, SNIP, mKIAA1684, p140Cap, Snip
Epitope
N-Term
(6), (2), (1)
Reactivity
Rat (Rattus), Mouse (Murine), Human
(11), (9), (9)
Host
Rabbit
(11)
Clonality
Polyclonal
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(11), (7), (1)
Pubmed 5 references available
Quantity 100 μL
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Catalog No. ABIN350837
454.67 $
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Immunogen A synthetic peptide from the n-terminal of human SNIP conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Specificity Specific for SNIP.
Purification Whole serum
Alternative Name SNIP
Background Function: Delays the onset of cell spreading in the early stages of cell adhesion to fibronectin. Also involved in calcium-dependent exocytosis from PC12 cells.
Subunit: Interacts with the N-terminal coiled-coil region ofSNAP25. Interacts with BCAR1/p130Cas through its C-terminal domain.
Subcellular location: Cytoplasm. Cytoplasm, cytoskeleton. Note: Localized to the perinuclear region, lamellopodia, cortical actin and actin stress fibers but not to focal adhesions. Also known as: p130 Cas associated protein, p140 Cap,SNAP 25 interacting protein, P140.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (N-Term) antibody IF on 1 day- old mouse cortical cell culture using Rabbit antibody to n-terminal of SNIP (SNAP 25 interacting protein): whole serum (ABIN350837) at 1: 2000 dilution. DAPI counter stained.
anti-SRC Kinase Signaling Inhibitor 1 (SRCIN1) (N-Term) antibody (2) IF on 1 day- old mouse cortical cell culture using Rabbit antibody to n-terminal of SNIP (SNAP 25 interacting protein): whole serum (ABIN350837) at 1: 2000 dilution. DAPI counter stained appearing in blue.
General Nagase, Kikuno, Hattori et al.: "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 6, pp. 347-55, 2001 (PubMed).

Di Stefano, Cabodi, Boeri Erba et al.: "P130Cas-associated protein (p140Cap) as a new tyrosine-phosphorylated protein involved in cell spreading." in: Molecular biology of the cell, Vol. 15, Issue 2, pp. 787-800, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

DeGiorgis, Jaffe, Moreira et al.: "Phosphoproteomic analysis of synaptosomes from human cerebral cortex." in: Journal of proteome research, Vol. 4, Issue 2, pp. 306-15, 2005 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).

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