SOD3 (Superoxide dismutase 3, SODE) (N-Term) antibody

Antigen: SOD3 (Superoxide dismutase 3, SODE)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350843

Additional Information

UniProt: P08294

Immunogen: A synthetic peptide from the n-terminal region of human SOD3 (Superoxide dismutase 3, SODE) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Function: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Catalytic activity: 2 superoxide + 2 H(+) = O(2) + H(2)O(2). Tissue specificity: Expressed in blood vessels, heart, lungs, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid. Subcellular location: Secreted › extracellular space. Note: 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium. Also known as: Superoxide dismutase 3, SODE, SOD E.

Specificity: Appears to be specific for SOD3.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Adachi, Ohta, Hayashi et al.: "The site of nonenzymic glycation of human extracellular-superoxide dismutase in vitro." in: Free radical biology & medicine, Vol. 13, Issue 3, pp. 205-10, 1992 (PubMed).

Hjalmarsson, Marklund, Engstroem et al.: "Isolation and sequence of complementary DNA encoding human extracellular superoxide dismutase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 18, pp. 6340-4, 1987 (PubMed).

Folz, Crapo: "Extracellular superoxide dismutase (SOD3): tissue-specific expression, genomic characterization, and computer-assisted sequence analysis of the human EC SOD gene." in: Genomics, Vol. 22, Issue 1, pp. 162-71, 1994 (PubMed).

Adachi, Morihara, Yamazaki et al.: "An arginine-213 to glycine mutation in human extracellular-superoxide dismutase reduces susceptibility to trypsin-like proteinases." in: Journal of biochemistry, Vol. 120, Issue 1, pp. 184-8, 1997 (PubMed).

Nozik-Grayck, Suliman, Piantadosi: "Extracellular superoxide dismutase." in: The international journal of biochemistry & cell biology, Vol. 37, Issue 12, pp. 2466-71, 2005 (PubMed).