Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 1 (SLC9A1) (cytoplasmic domain) antibody

Antigen: Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 1 (SLC9A1)

Synonyms: APNH, NHE1, FLJ42224, swe, Apnh, Nhe1, AW554487, SLC9A1, AT-NHX1, ATNHX, ATNHX1, NA(+)/H(+) ANTIPORTER, Na+/H+ exchanger 1, NHX1, T21B4.60, T21B4_60

Epitope: cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350847

Additional Information

Alternative name: Sodium / Hydrogen Exchanger 1 (SLC9A1, NHE1) (cytoplasmic domain)

Swiss-Prot: P19634

Immunogen: A synthetic peptide from the 7th cytoplasmic domain of Sodium / Hydrogen Exchanger 1 (SLC9A1, NHE1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Subcellular location: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Kidney and intestine. Also known as: Sodium/hydrogen exchanger 1, Na(+)/H(+) exchanger 1, NHE-1, NHE1, Solute carrier family 9 member 1, Na(+)/H(+) antiporter, amiloride-sensitive, APNH, SLC9A1.

Specificity: Appears to be specific for SL9A1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Sardet, Franchi, Pouyssuegur: "Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter." in: Cell, Vol. 56, Issue 2, pp. 271-80, 1989 (PubMed).

Counillon, Pouyssuegur, Reithmeier: "The Na+/H+ exchanger NHE-1 possesses N- and O-linked glycosylation restricted to the first N-terminal extracellular domain." in: Biochemistry, Vol. 33, Issue 34, pp. 10463-9, 1994 (PubMed).

Garden, Musk, Worthington-White et al.: "Silent polymorphisms within the coding region of human sodium/hydrogen exchanger isoform-1 cDNA in peripheral blood mononuclear cells of leukemia patients: A comparison with healthy controls." in: Cancer genetics and cytogenetics, Vol. 120, Issue 1, pp. 37-43, 2000 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Mishima, Wakabayashi, Kojima: "Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1." in: The Journal of biological chemistry, Vol. 282, Issue 4, pp. 2741-51, 2007 (PubMed).