Sodium Channel, Voltage-Gated, Type IV, alpha Subunit (SCN4A) (N-Term) antibody

Antigen: Sodium Channel, Voltage-Gated, Type IV, alpha Subunit (SCN4A)

Synonyms: HYPP, SkM1, HYKPP, NAC1A, Nav1.4, Na(V)1.4, NCHVS, microI

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350854

Additional Information

Alternative name: Sodium channel protein type 4 subunitalpha (SCN4A, Nav1.4) (n-terminal)

Swiss-Prot: Q9ER60

Immunogen: A synthetic peptide from the n-terminal of mouse Sodium channel protein type 4 subunit alpha (SCN4A, Nav1.4) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and rat.

Format: Lyophilized

Description: FUNCTION: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Also known as: Sodium channel protein type IV subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.4, Sodium channel protein skeletal muscle subunit alpha, SkM1, SCN4A.

Specificity: Appears to be specific for SCN4A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Wang, Rojas, Zhou et al.: "Sequence and genomic structure of the human adult skeletal muscle sodium channel alpha subunit gene on 17q." in: Biochemical and biophysical research communications, Vol. 182, Issue 2, pp. 794-801, 1992 (PubMed).

McClatchey, Van den Bergh, Pericak-Vance et al.: "Temperature-sensitive mutations in the III-IV cytoplasmic loop region of the skeletal muscle sodium channel gene in paramyotonia congenita." in: Cell, Vol. 68, Issue 4, pp. 769-74, 1992 (PubMed).

George, Komisarof, Kallen et al.: "Primary structure of the adult human skeletal muscle voltage-dependent sodium channel." in: Annals of neurology, Vol. 31, Issue 2, pp. 131-7, 1992 (PubMed).

Ptuacek, George, Barchi et al.: "Mutations in an S4 segment of the adult skeletal muscle sodium channel cause paramyotonia congenita." in: Neuron, Vol. 8, Issue 5, pp. 891-7, 1992 (PubMed).

Luan, Chen, Liu et al.: "Tubular aggregates in paralysis periodica paramyotonica with T704M mutation of SCN4A." in: Neuropathology : official journal of the Japanese Society of Neuropathology, Vol. 29, Issue 5, pp. 579-84, 2009 (PubMed).