Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A) (internal) antibody

Antigen: Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A)

Synonyms: HB1, HB2, HH1, IVF, VF1, HBBD, ICCD, LQT3, SSS1, CDCD2, CMD1E, CMPD2, PFHB1, Nav1.5, mH1, SkM1, Nav1.5c, SCAL, SCN5A

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350855

Additional Information

Alternative name: Sodium channel protein type 5 subunitalpha (SCN5A, Nav1.5) (internal region)

Swiss-Prot: Q14524

Immunogen: A synthetic peptide from the internal region of human Sodium channel protein type 5 subunit alpha (SCN5A, Nav1.5) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na+ channel isoform. This channel is responsible for the initial upstroke of the action potential in the electrocardiogram. Tissue specificity: Found in jejunal circular smooth muscle cells (at protein level). Expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. Isoform 4 is expressed in brain. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Also known as: Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, Sodium channel protein cardiac muscle subunit alpha, HH1, SCN5A.

Specificity: Appears to be specific for SCN5A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Gellens, George, Chen et al.: "Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 554-8, 1992 (PubMed).

Vatta, Dumaine, Varghese et al.: "Genetic and biophysical basis of sudden unexplained nocturnal death syndrome (SUNDS), a disease allelic to Brugada syndrome." in: Human molecular genetics, Vol. 11, Issue 3, pp. 337-45, 2002 (PubMed).

Makielski, Ye, Valdivia et al.: "A ubiquitous splice variant and a common polymorphism affect heterologous expression of recombinant human SCN5A heart sodium channels." in: Circulation research, Vol. 93, Issue 9, pp. 821-8, 2003 (PubMed).

van Bemmelen, Rougier, Gavillet et al.: "Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2 mediated ubiquitination." in: Circulation research, Vol. 95, Issue 3, pp. 284-91, 2004 (PubMed).

Ou, Kameyama, Hao et al.: "Tetrodotoxin-resistant Na+ channels in human neuroblastoma cells are encoded by new variants of Nav1.5/SCN5A." in: The European journal of neuroscience, Vol. 22, Issue 4, pp. 793-801, 2005 (PubMed).