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Sodium Channel, Voltage-Gated, Type V, alpha Subunit (SCN5A) (Internal Region) antibody

Details for Product No. ABIN350855, Supplier: Log in to see
Antigen
  • CDCD2
  • CMD1E
  • CMPD2
  • HB1
  • HB2
  • HBBD
  • HH1
  • ICCD
  • IVF
  • LQT3
  • mH1
  • Nav1.5
  • Nav1.5c
  • PFHB1
  • SCAL
  • SkM1
  • SkM2
  • SSS1
  • VF1
Epitope
Internal Region
27
19
17
5
2
1
Reactivity
Human
106
13
13
8
6
2
1
1
1
1
1
1
Host
Rabbit
41
37
29
Clonality
Polyclonal
Conjugate
Un-conjugated
6
6
6
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
74
48
19
19
18
12
7
2
1
Supplier
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Immunogen A synthetic peptide from the internal region of human Sodium channel protein type 5 subunit alpha (SCN5A, Nav1.5) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for SCN5A.
Purification Whole serum
Alternative Name SCN5A (SCN5A Antibody Abstract)
Background Function: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na+ channel isoform. This channel is responsible for the initial upstroke of the action potential in the electrocardiogram.
Tissue specificity: Found in jejunal circular smooth muscle cells (at protein level). Expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. Isoform 4 is expressed in brain.
Subcellular location: Cell membrane, Multi-pass membrane protein. Also known as: Sodium channel protein type V subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.5, Sodium channel protein cardiac muscle subunit alpha, HH1, SCN5A.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Ou, Kameyama, Hao, Horiuchi, Minobe, Wang, Makita, Kameyama: "Tetrodotoxin-resistant Na+ channels in human neuroblastoma cells are encoded by new variants of Nav1.5/SCN5A." in: The European journal of neuroscience, Vol. 22, Issue 4, pp. 793-801, 2005

van Bemmelen, Rougier, Gavillet, Apothueloz, Daidiue, Tateyama, Rivolta, Thomas, Kass, Staub, Abriel: "Cardiac voltage-gated sodium channel Nav1.5 is regulated by Nedd4-2 mediated ubiquitination." in: Circulation research, Vol. 95, Issue 3, pp. 284-91, 2004

Makielski, Ye, Valdivia, Pagel, Pu, Tester, Ackerman: "A ubiquitous splice variant and a common polymorphism affect heterologous expression of recombinant human SCN5A heart sodium channels." in: Circulation research, Vol. 93, Issue 9, pp. 821-8, 2003

Vatta, Dumaine, Varghese, Richard, Shimizu, Aihara, Nademanee, Brugada, Brugada, Veerakul, Li, Bowles, Brugada, Antzelevitch, Towbin: "Genetic and biophysical basis of sudden unexplained nocturnal death syndrome (SUNDS), a disease allelic to Brugada syndrome." in: Human molecular genetics, Vol. 11, Issue 3, pp. 337-45, 2002

Gellens, George, Chen, Chahine, Horn, Barchi, Kallen: "Primary structure and functional expression of the human cardiac tetrodotoxin-insensitive voltage-dependent sodium channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 554-8, 1992