Sodium Channel, Voltage-Gated, Type VIII, alpha (SCN8A) (internal) antibody

Antigen: Sodium Channel, Voltage-Gated, Type VIII, alpha (SCN8A)

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350858

Additional Information

Alternative name: Sodium channel protein type 8 subunitalpha (SCN8A, Nav1.6) (internal region)

Swiss-Prot: Q9WTU3

Immunogen: A synthetic peptide from the internal region of mouse Sodium channel protein type 8 subunit alpha (SCN8A, Nav1.6) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and human.

Format: Lyophilized

Description: FUNCTION: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. Tissue specificity: Expressed in brain, cerebellum and spinal cord. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Also known as: Sodium channel protein type VIII subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.6, SCN8A, MED, Nav1.6.

Specificity: Appears to be specific for SCN8A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Schaller, Krzemien, Yarowsky et al.: "A novel, abundant sodium channel expressed in neurons and glia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 5 Pt 1, pp. 3231-42, 1995 (PubMed).

Plummer, McBurney, Meisler: "Alternative splicing of the sodium channel SCN8A predicts a truncated two-domain protein in fetal brain and non-neuronal cells." in: The Journal of biological chemistry, Vol. 272, Issue 38, pp. 24008-15, 1997 (PubMed).

Plummer, Galt, Jones et al.: "Exon organization, coding sequence, physical mapping, and polymorphic intragenic markers for the human neuronal sodium channel gene SCN8A." in: Genomics, Vol. 54, Issue 2, pp. 287-96, 1999 (PubMed).

De Repentigny, Cuotue, Pool et al.: "Pathological and genetic analysis of the degenerating muscle (dmu) mouse: a new allele of Scn8a." in: Human molecular genetics, Vol. 10, Issue 17, pp. 1819-27, 2001 (PubMed).

Fotia, Ekberg, Adams et al.: "Regulation of neuronal voltage-gated sodium channels by the ubiquitin-protein ligases Nedd4 and Nedd4-2." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28930-5, 2004 (PubMed).