Sodium channel protein type 9 subunitalpha (SCN9A, Nav1.7) (C-Term) antibody

Antigen: Sodium channel protein type 9 subunitalpha (SCN9A, Nav1.7)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350859

Additional Information

UniProt: Q15858

Immunogen: A synthetic peptide from the c-terminal region of human Sodium channel protein type 9 subunit alpha (SCN9A, Nav1.7) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na+ channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain. Tissue specificity: Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system while isoform 2 is expressed preferentially in the dorsal root ganglion. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note: In neurite terminals Also known as: Sodium channel protein type IX subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.7, Neuroendocrine sodium channel, Peripheral sodium channel 1, SCN9A, NENA, PN1.

Specificity: Appears to be specific for SCN9A.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Sangameswaran, Fish, Koch et al.: "A novel tetrodotoxin-sensitive, voltage-gated sodium channel expressed in rat and human dorsal root ganglia." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 14805-9, 1997 (PubMed).

Yang, Wang, Li et al.: "Mutations in SCN9A, encoding a sodium channel alpha subunit, in patients with primary erythermalgia." in: Journal of medical genetics, Vol. 41, Issue 3, pp. 171-4, 2004 (PubMed).

Raymond, Castle, Garrett-Engele et al.: "Expression of alternatively spliced sodium channel alpha-subunit genes. Unique splicing patterns are observed in dorsal root ganglia." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46234-41, 2004 (PubMed).

Fertleman, Baker, Parker et al.: "SCN9A mutations in paroxysmal extreme pain disorder: allelic variants underlie distinct channel defects and phenotypes." in: Neuron, Vol. 52, Issue 5, pp. 767-74, 2006 (PubMed).

Cox, Reimann, Nicholas et al.: "An SCN9A channelopathy causes congenital inability to experience pain." in: Nature, Vol. 444, Issue 7121, pp. 894-8, 2006 (PubMed).