Sortilin (Neurotensin receptor 3, NTR3, Sort1) (Cytoplasmic Domain) antibody

Antigen: Sortilin (Neurotensin receptor 3, NTR3, Sort1)

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350865

Additional Information

UniProt: Q99523

Immunogen: A synthetic peptide from the cytoplasmic domain of human Sortilin (Neurotensin receptor 3, NTR3, Sort1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP and GM2A, LPL, PSAP and SLC2A4. Forms a complex with NGFR which binds specifically to the precursor form of NGFB (proNGFB). Interacts with the precursor form of BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note: Localized to membranes of the endosomes, Golgi stack, lysosomes and nucleus. Localization to the plasma membrane in adipocytes is enhanced by insulin. Localized to the endoplasmic reticulum and the plasma membrane. Also found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver. Also known as: Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95, 100 kDa NT receptor.

Specificity: Appears to be specific for Sortilin.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Petersen, Nielsen, Nykjaer et al.: "Molecular identification of a novel candidate sorting receptor purified from human brain by receptor-associated protein affinity chromatography." in: The Journal of biological chemistry, Vol. 272, Issue 6, pp. 3599-605, 1997 (PubMed).

Mazella, Zsürger, Navarro et al.: "The 100-kDa neurotensin receptor is gp95/sortilin, a non-G-protein-coupled receptor." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26273-6, 1998 (PubMed).

Nielsen, Jacobsen, Olivecrona et al.: "Sortilin/neurotensin receptor-3 binds and mediates degradation of lipoprotein lipase." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8832-6, 1999 (PubMed).

Nielsen, Madsen, Christensen et al.: "The sortilin cytoplasmic tail conveys Golgi-endosome transport and binds the VHS domain of the GGA2 sorting protein." in: The EMBO journal, Vol. 20, Issue 9, pp. 2180-90, 2001 (PubMed).

Shiba, Takatsu, Nogi et al.: "Structural basis for recognition of acidic-cluster dileucine sequence by GGA1." in: Nature, Vol. 415, Issue 6874, pp. 937-41, 2002 (PubMed).

Maeda, Nobukuni, Shimo-Onoda et al.: "Sortilin is upregulated during osteoblastic differentiation of mesenchymal stem cells and promotes extracellular matrix mineralization." in: Journal of cellular physiology, Vol. 193, Issue 1, pp. 73-9, 2002 (PubMed).

Martin, Vincent, Mazella: "Involvement of the neurotensin receptor-3 in the neurotensin-induced migration of human microglia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 4, pp. 1198-205, 2003 (PubMed).

Teng, Teng, Lee et al.: "ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 22, pp. 5455-63, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Zahedi, Lewandrowski, Wiesner et al.: "Phosphoproteome of resting human platelets." in: Journal of proteome research, Vol. 7, Issue 2, pp. 526-34, 2008 (PubMed).