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Sortilin (Neurotensin receptor 3, NTR3, Sort1) (Extracellular Domain, N-Term) antibody
Extracellular Domain, N-Term
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
|Quantity||100 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Immunogen||A synthetic peptide from the extracellular, N-terminal part of rat Sortilin (Neurotensin receptor 3, NTR3, Sort1) conjugated to immunogenic carrier protein was used as the immunogen. The antigen shares 95% identity with mouse's.|
|Description||FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP and GM2A, LPL, PSAP and SLC2A4. Forms a complex with NGFR which binds specifically to the precursor form of NGFB (proNGFB). Interacts with the precursor form of BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Note: Localized to membranes of the endosomes, Golgi stack, lysosomes and nucleus. Localization to the plasma membrane in adipocytes is enhanced by insulin. Localized to the endoplasmic reticulum and the plasma membrane. Also found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. TISSUE SPECIFICITY: Highly expressed in fat, brain, and lung. Expressed in neuronal bodies and dendrites of the piriform cortex and hippocampus. Also expressed in the islands of Calleja, medial and lateral septal nuclei, amygdaloid nuclei, thalamic nuclei, the supraoptic nucleus, the substantia nigra, the Purkinje layer of the cerebellar cortex and the cranial motor nerve nuclei of the brainstem. Also known as: Neurotensin receptor 3, NTR3, Glycoprotein 110, Gp110, Nt3, Nts3.|
|Specificity||Appears to be specific for Sortilin.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 3000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Lin, Pilch, Kandror: "Sortilin is a major protein component of Glut4-containing vesicles." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24145-7, 1997 (PubMed).
Morris, Ross, Lane et al.: "Sortilin is the major 110-kDa protein in GLUT4 vesicles from adipocytes." in: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3582-7, 1998 (PubMed).
Sarret, Krzywkowski, Segal et al.: "Distribution of NTS3 receptor/sortilin mRNA and protein in the rat central nervous system." in: The Journal of comparative neurology, Vol. 461, Issue 4, pp. 483-505, 2003 (PubMed).
Conticello, Kowalsman, Jacobsen et al.: "The prodomain of a secreted hydrophobic mini-protein facilitates its export from the endoplasmic reticulum by hitchhiking on sorting receptors." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26311-4, 2003 (PubMed).
Gibbs, Weinstock, Metzker et al.: "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." in: Nature, Vol. 428, Issue 6982, pp. 493-521, 2004 (PubMed).
Chen, Ieraci, Teng et al.: "Sortilin controls intracellular sorting of brain-derived neurotrophic factor to the regulated secretory pathway." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 26, pp. 6156-66, 2005 (PubMed).
|Hosts||Rabbit (6), Sheep (2)|
|Applications||Immunohistochemistry (IHC) (8), Western Blotting (WB) (8)|
|Epitopes||Extracellular Domain, N-Term (5), Cytoplasmic Domain (2), Extracellular Domain, C-Term (1)|