Sorting nexin 2 (Nexin-2, SNX2, TRG9) (C-Term) antibody

Antigen: Sorting nexin 2 (Nexin-2, SNX2, TRG9)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350874

Additional Information

UniProt: O60749

Immunogen: A synthetic peptide from the c-terminal of human Sorting nexin 2 (Nexin-2, SNX2, TRG9) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: May be involved in several stages of intracellular trafficking. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also known as: Nexin-2, SNX2, Transformation-related gene 9 protein, TRG-9, TRG9.

Specificity: Appears to be specific for Sorting nexin 2.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Haft, de la Luz Sierra, Barr et al.: "Identification of a family of sorting nexin molecules and characterization of their association with receptors." in: Molecular and cellular biology, Vol. 18, Issue 12, pp. 7278-87, 1998 (PubMed).

Fuchs, Rehkamp, Haas et al.: "The human formin-binding protein 17 (FBP17) interacts with sorting nexin, SNX2, and is an MLL-fusion partner in acute myelogeneous leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 15, pp. 8756-61, 2001 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).