STAM 1, 2 (STAM-1, STAM-2 (pan, Internal Region) antibody

Antigen: STAM 1, 2 (STAM-1, STAM-2

Binding Site: pan, Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350877

Additional Information

UniProt: Q92783

Immunogen: A synthetic peptide from the internal part of human STAM 1, 2 (STAM-1, STAM-2) conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous in many species including rat, mouse, zebra fish and xenopus.

Format: Lyophilized

Description: STAM1: FUNCTION: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and c-myc induction. May also play a role in endocytic trafficking as well as in T-cell development. SUBUNIT: Heterodimer. Composed of STAM1 and HGS/HRS. Also interacts directly with STAMBP/AMSH. TISSUE SPECIFICITY: Ubiquitously expressed. STAM2: FUNCTION: Involved in intracellular signal transduction mediated by cytokines and growth factors. Probably acts via its association with ubiquitinated cargo proteins on the early endosome participates in their sorting for trafficking to the lysosome. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and c-myc induction. May also play a in T-cell development. SUBUNIT: Heterodimer or heterotrimer. At least composed of STAM2 and HGS/Hrs. EPS15 can be part of the complex. Interacts directly with STAMBP/AMSH, JAK2 and JAK3. Also interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY. Interacts directly with CBX5 via the PxVxL motif. Interacts with VPS37C. TISSUE SPECIFICITY: Ubiquitously expressed. Also known as: STAM1, STAM-1, STAM2, STAM-2, Signal transducing adapter molecule 1, Signal transducing adapter molecule 2, HBP, Hrs-binding protein.

Specificity: Appears to be specific for STAM1 & 2.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit pan antibody to an internal part of STAM 1, 2 (STAM-1, STAM-2): whole serum (ABIN350877) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected bands.

Publications

Takeshita, Arita, Asao et al.: "Cloning of a novel signal-transducing adaptor molecule containing an SH3 domain and ITAM." in: Biochemical and biophysical research communications, Vol. 225, Issue 3, pp. 1035-9, 1996 (PubMed).

Asao, Sasaki, Arita et al.: "Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 32785-91, 1998 (PubMed).

Steen, Kuster, Fernandez et al.: "Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1031-9, 2002 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).