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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Stromal Interaction Molecule 1 (STIM1) (Cytoplasmic Domain) antibody
|Synonyms||GOK, D11S4896E, SIM, stim1, MGC76045, STIM1, MGC154748, si:ch211-112l17.2, Stim1|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||Stromal interaction molecule 1 (STIM1, GOK)|
|Immunogen||A synthetic peptide from the cytoplasmic domain of human Stromal interaction molecule 1 (STIM1) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION: Plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note: Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium. Tissue specificity: Ubiquitously expressed in various human primary cells and tumor cell lines. Also known as: STIM1, GOK.|
|Specificity||Appears to be specific for STIM1.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Parker, Begley, Smith et al.: "Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5." in: Genomics, Vol. 37, Issue 2, pp. 253-6, 1997 (PubMed).
Sabbioni, Barbanti-Brodano, Croce et al.: "GOK: a gene at 11p15 involved in rhabdomyosarcoma and rhabdoid tumor development." in: Cancer research, Vol. 57, Issue 20, pp. 4493-7, 1997 (PubMed).
Manji, Parker, Williams et al.: "STIM1: a novel phosphoprotein located at the cell surface." in: Biochimica et biophysica acta, Vol. 1481, Issue 1, pp. 147-55, 2000 (PubMed).
Williams, Manji, Parker et al.: "Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins." in: The Biochemical journal, Vol. 357, Issue Pt 3, pp. 673-85, 2001 (PubMed).
Chen, Jiang, Sun et al.: "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." in: Journal of proteome research, Vol. 8, Issue 2, pp. 651-61, 2009 (PubMed).
|Hosts||Rabbit (41), Mouse (10), Goat (3), Sheep (1)|
|Reactivities||Human (47), Rat (Rattus) (31), Mouse (Murine) (30), Dog (Canine) (21), Cow (Bovine) (19), Chicken (18), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18)|
|Applications||Western Blotting (WB) (39), ELISA (22), Immunofluorescence (IF) (19), Flow Cytometry (FACS) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunohistochemistry (IHC) (11), Immunoprecipitation (IP) (7), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||C-Term (10), Extracellular Domain (4), Internal Region (2), AA 600-685 (1)|