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Stromal Interaction Molecule 1 (STIM1) (Extracellular Domain), (AA 50-100) antibody

Details for Product No. ABIN350880
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Antigen
Synonyms stim1, MGC76045, STIM1, si:dkey-24p1.5, MGC154748, si:ch211-112l17.2, Stim1, D11S4896E, GOK, TAM, SIM
Epitope
Extracellular Domain, AA 50-100
(27), (9), (8), (6), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(80), (46), (46), (25), (24), (18), (17), (2)
Host
Rabbit
(59), (22), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(70), (32), (24), (21), (14), (11), (10), (7), (2), (1)
Pubmed 5 references available
Quantity 100 µL
Options
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Catalog No. ABIN350880
454.67 $
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Immunogen A synthetic peptide from aa region 50-100 of extracellular domain of human STIM1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
Specificity Specific for STIM1.
Purification Whole serum
Alternative Name STIM1
Background Function: Plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1.
Subcellular location: Cell membrane, Single-pass type I membrane protein. Endoplasmic reticulum membrane, Single-pass type I membrane protein. Note: Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium.
Tissue specificity: Ubiquitously expressed in various human primary cells and tumor cell lines. Also known as: STIM1, GOK.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-Stromal Interaction Molecule 1 (STIM1) (Extracellular Domain), (AA 50-100) antibody WB on mouse brain lysate (denatured, reduced) using Rabbit antibody to extracellular domain of Stromal interaction molecule 1 (STIM1, GOK): whole serum (ABIN350880) at 1: 1000 dilution. Blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
General Parker, Begley, Smith et al.: "Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5." in: Genomics, Vol. 37, Issue 2, pp. 253-6, 1997 (PubMed).

Sabbioni, Barbanti-Brodano, Croce et al.: "GOK: a gene at 11p15 involved in rhabdomyosarcoma and rhabdoid tumor development." in: Cancer research, Vol. 57, Issue 20, pp. 4493-7, 1997 (PubMed).

Manji, Parker, Williams et al.: "STIM1: a novel phosphoprotein located at the cell surface." in: Biochimica et biophysica acta, Vol. 1481, Issue 1, pp. 147-55, 2000 (PubMed).

Williams, Manji, Parker et al.: "Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins." in: The Biochemical journal, Vol. 357, Issue Pt 3, pp. 673-85, 2001 (PubMed).

Chen, Jiang, Sun et al.: "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." in: Journal of proteome research, Vol. 8, Issue 2, pp. 651-61, 2009 (PubMed).

Validation Images
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