Stromal Interaction Molecule 2 (Stim2) (extracellular domain) antibody

Antigen: Stromal Interaction Molecule 2 (Stim2)

Synonyms: FLJ39527, KIAA1482, STIM2

Epitope: extracellular domain

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350882

Additional Information

Alternative name: Stromal interaction molecule 2 (STIM2) (extracellular domain)

Swiss-Prot: P83093

Immunogen: A synthetic peptide from the extracellular domain of mouse Stromal interaction molecule 1 (STIM2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and human.

Format: Lyophilized

Description: FUNCTION: Functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca2+ influx. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein, Endoplasmic reticulum membrane; Single-pass type I membrane. Note: Translocates from the endoplasmic reticulum to punctual endoplasmic reticulum-cell plasma membrane junctions in response to decrease in endoplasmic reticulum Ca2+ concentration. Tissue specificity: Expressed in all tissues and tumor cell lines examined. Also known as: STIM2.

Specificity: Appears to be specific for STIM2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Williams, Manji, Parker et al.: "Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins." in: The Biochemical journal, Vol. 357, Issue Pt 3, pp. 673-85, 2001 (PubMed).

Okazaki, F-Kikuno, Ohara et al.: "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 11, Issue 3, pp. 205-18, 2004 (PubMed).

Villuen, Beausoleil, Gerber et al.: "Large-scale phosphorylation analysis of mouse liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 5, pp. 1488-93, 2007 (PubMed).

Dephoure, Zhou, Villuen et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Stathopulos, Zheng, Ikura: "Stromal interaction molecule (STIM) 1 and STIM2 calcium sensing regions exhibit distinct unfolding and oligomerization kinetics." in: The Journal of biological chemistry, Vol. 284, Issue 2, pp. 728-32, 2009 (PubMed).