SUMO 1 (SUMO-1, Small ubiquitin related modifier 1) (N-Term) antibody

Antigen: SUMO 1 (SUMO-1, Small ubiquitin related modifier 1)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350883

Additional Information

UniProt: Q5I0H3

Immunogen: A synthetic peptide from the n-terminal of rat SUMO 1 (SUMO-1, Small ubiquitin related modifier 1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human, mouse and chicken.

Format: Lyophilized

Description: Function: Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 Subcellular location: Nucleus. Cytoplasm Post-translational modification: Cleavage of precursor form by SENP1 or SENP2 is necessary for function. Also known as: Small ubiquitin-related modifier 1, SUMO-1, Sumo1.

Specificity: Appears to be specific for SUMO 1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Shao, Zhang, Tian et al.: "Increase of SUMO-1 expression in response to hypoxia: direct interaction with HIF-1alpha in adult mouse brain and heart in vivo." in: FEBS letters, Vol. 569, Issue 1-3, pp. 293-300, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Um, Chung: "Functional modulation of parkin through physical interaction with SUMO-1." in: Journal of neuroscience research, Vol. 84, Issue 7, pp. 1543-54, 2006 (PubMed).