Synapsin III (SYN3) antibody

Antigen: Synapsin III (SYN3)

Synonyms: SYN3, MGC130403, syn3-A

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350892

Additional Information

Alternative name: Synapsin-3 (Synapsin 3, Syn3,Synapsin III)

Swiss-Prot: O70441

Immunogen: A synthetic peptide as a part of rat, mouse Synapsin-3 (Synapsin 3, Syn3, Synapsin III) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: May be involved in the regulation of neurotransmitter release and synaptogenesis. Binds ATP with high affinity and ADP with a lower affinity. This is consistent with a catalytic role of the C-domain in which ADP would be dissociated by cellular ATP after bound ATP was hydrolyzed. SUBUNIT: Interacts with CAPON. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Peripheral membrane protein localized to the cytoplasmic surface of synaptic vesicles. MISCELLANEOUS: Regulated by calcium. Calcium inhibits ATP binding to the C-domain. Also known as: Synapsin 3, Syn3, Synapsin III.

Specificity: Appears to be specific for Synapsin-3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IHC on rat sciatic nerve using Rabbit antibody to rat, mouse Synapsin-3 (Synapsin 3, Syn3, Synapsin III): whole serum (ABIN350892) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publications

Publications: Hosaka, Suedhof: "Synapsin III, a novel synapsin with an unusual regulation by Ca2+." in: The Journal of biological chemistry, Vol. 273, Issue 22, pp. 13371-4, 1998 (PubMed).

Ehringer, Thompson, Conroy et al.: "High-throughput sequence identification of gene coding variants within alcohol-related QTLs." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 12, Issue 8, pp. 657-63, 2001 (PubMed).

Jaffrey, Benfenati, Snowman et al.: "Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3199-204, 2002 (PubMed).

Trinidad, Specht, Thalhammer et al.: "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." in: Molecular & cellular proteomics : MCP, Vol. 5, Issue 5, pp. 914-22, 2006 (PubMed).

Munton, Tweedie-Cullen, Livingstone-Zatchej et al.: "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 2, pp. 283-93, 2007 (PubMed).