Synaptophysin (SYP) (internal) antibody

Antigen: Synaptophysin (SYP)

Synonyms: Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350895

Additional Information

Alternative name: Synaptophysin (SYP) (internal region)

Swiss-Prot: P07825

Immunogen: A synthetic peptide from rat Synaptophysin (SYP) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Description: Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. FUNCTION: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. SUBUNIT: Homohexamer or homotetramer. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Also known as: SYP.

Specificity: Appears to be specific for Synaptophysin.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Synaptophysin (SYP): whole serum (ABIN350895) at 1: 2000 dilution. DAPI counter stained.

IHC on rat trigeminal nerve using Rabbit antibody to rat, mouse Synaptophysin (SYP): whole serum (ABIN350895) at 1 : 2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

WB on rat brain lysate using IHC on rat trigeminal nerve using Rabbit antibody to rat, mouse Synaptophysin (SYP): whole serum (ABIN350895) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Bargou, Leube: "The synaptophysin-encoding gene in rat and man is specifically transcribed in neuroendocrine cells." in: Gene, Vol. 99, Issue 2, pp. 197-204, 1991 (PubMed).

Suedhof, Lottspeich, Greengard et al.: "The cDNA and derived amino acid sequences for rat and human synaptophysin." in: Nucleic acids research, Vol. 15, Issue 22, pp. 9607, 1988 (PubMed).

Suedhof, Lottspeich, Greengard et al.: "A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions." in: Science (New York, N.Y.), Vol. 238, Issue 4830, pp. 1142-4, 1987 (PubMed).

Buckley, Floor, Kelly: "Cloning and sequence analysis of cDNA encoding p38, a major synaptic vesicle protein." in: The Journal of cell biology, Vol. 105, Issue 6 Pt 1, pp. 2447-56, 1988 (PubMed).

Leube, Kaiser, Seiter et al.: "Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA." in: The EMBO journal, Vol. 6, Issue 11, pp. 3261-8, 1988 (PubMed).

Wheeler, Chin, Li et al.: "Regulation of synaptophysin degradation by mammalian homologues of seven in absentia." in: The Journal of biological chemistry, Vol. 277, Issue 12, pp. 10273-82, 2002 (PubMed).