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SNAP25 antibody (Synaptosomal-Associated Protein, 25kDa)

Details for Product anti-SNAP25 Antibody No. ABIN350906, Supplier: Log in to see
Antigen
  • bA416N4.2
  • Bdr
  • dJ1068F16.2
  • fj55h11
  • ric-4
  • RIC-4
  • ric4
  • RIC4
  • SEC9
  • sec9
  • SNAP
  • SNAP-25
  • snap-25
  • SNAP-25a
  • SNAP-25B
  • snap25
  • sp
  • wu:fj55h11
Reactivity
Human
307
136
105
51
29
21
16
15
14
8
6
5
3
3
2
2
1
1
1
1
1
Host
Rabbit
169
143
25
6
1
Clonality
Polyclonal
Conjugate
This SNAP25 antibody is un-conjugated
13
11
9
9
7
7
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
272
190
98
69
68
47
28
18
15
12
12
9
2
2
2
1
1
Supplier
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Supplier Product No.
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Immunogen A synthetic peptide as a part of human Synaptosomal-associated protein 25 (SNAP-25, SNAP25, SEC9, RIC-4) conjugated to an immunogenic carrier protein was used as the immunogen.
Isotype IgG
Specificity Specific for SNAP-25.
Alternative Name SNAP 25 (SNAP25 Antibody Abstract)
Background Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.
Subunit: Part of the SNARE core complex containingSNAP25, VAMP2 and STX1A. This complex binds CPLX1. Interacts with TRIM9, RIMS1,SNAP25BP and HGS. Binds STXBP6. Found in a ternary complex with STX1A and VAMP8. Found in a complex containing SYT1, SV2B and syntaxin-1.
Subcellular location: Membrane, Lipid-anchor. Cell junction, synapse, synaptosome. Note: Membrane association requires palmitoylation.
Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Also known as:SNAP-25, Synaptosomal-associated 25 kDa protein,SNAP25, RIC4, SEC9,SNAP, RIC-4, Super protein, SUP, FLJ23079, bA416N4.2, dJ1068F16.2.
Application Notes A working concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Chen, Tomchick, Kovrigin, Araç, Machius, Südhof, Rizo: "Three-dimensional structure of the complexin/SNARE complex." in: Neuron, Vol. 33, Issue 3, pp. 397-409, 2002 (PubMed).

Jagadish, Fernandez, Hewish, Macaulay, Gough, Grusovin, Verkuylen, Cosgrove, Alafaci, Frenkel, Ward: "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." in: The Biochemical journal, Vol. 317 ( Pt 3), pp. 945-54, 1996 (PubMed).

Bark, Wilson: "Human cDNA clones encoding two different isoforms of the nerve terminal protein SNAP-25." in: Gene, Vol. 139, Issue 2, pp. 291-2, 1994 (PubMed).

Zhao, Hashida, Takahashi, Sakaki: "Cloning and sequence analysis of the human SNAP25 cDNA." in: Gene, Vol. 145, Issue 2, pp. 313-4, 1994 (PubMed).