Synaptosomal-Associated Protein, 29kD (SNAP29) antibody

Antigen: Synaptosomal-Associated Protein, 29kD (SNAP29)

Synonyms: CEDNIK, SNAP-29, FLJ21051, Gs32, AI891940, AU020222, BB131856, 1300018G05Rik, MGC105273, SNAP29, im:7145101, wu:fc84f09, DKFZp469O1214

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350910

Additional Information

Alternative name: Synaptosomal-associated protein 29 (SNAP-29,SNAP29, Golgi SNARE of 32 kDa)

Swiss-Prot: Q9Z2P6

Immunogen: A synthetic peptide from rat Synaptosomal-associated protein 29 (SNAP-29, Snap29, Golgi SNARE of 32 kDa) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: SUBUNIT: Binds tightly to multiple syntaxins. Preferentially binds to syntaxin 6. SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Note: Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. Binds withSNAP25 to form complexin. TISSUE SPECIFICITY: Widely expressed. Also known as:SNAP-29, Vesicle-membrane fusion protein,SNAP29, Soluble 29 kDa NSF attachment protein, Golgi SNARE of 32 kDa, Gs32.

Specificity: Appears to be specific for SNAP-29.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat Synaptosomal-associated protein 29 (SNAP-29, Snap29, Golgi SNARE of 32 kDa): whole serum (ABIN350910) at 1: 2000 dilution. DAPI counter stained.

Publications

Publications: Wong, Xu, Zhang et al.: "GS32, a novel Golgi SNARE of 32 kDa, interacts preferentially with syntaxin 6." in: Molecular biology of the cell, Vol. 10, Issue 1, pp. 119-34, 1999 (PubMed).

Pabst, Hazzard, Antonin et al.: "Selective interaction of complexin with the neuronal SNARE complex. Determination of the binding regions." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19808-18, 2000 (PubMed).