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Synapstotagmin I (SYT1) (N-Term Synaptotagmin I SytI p65 SVP65) antibody
| Antigen | Synapstotagmin I (SYT1) |
| Synonyms | P65, SYT, SVP65, DKFZp781D2042, AW124717, G630098F17Rik, syt, svp65, DKFZP459P193, SYT1, SytI, p65 |
| Epitope |
 Alternatives N-Term Synaptotagmin I SytI p65 SVP65 |
| Clonality | Polyclonal |
| Host |
Alternatives Rabbit |
| Application |
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|
 |
7 references available |
| Catalog no. | ABIN350919 |
| Quantity | 100 µl (Variants) |
| Price | 413.33 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65) (n-terminal region) |
| Swiss-Prot | P21707 |
| Immunogen | A synthetic peptide from rat Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse. |
| Format | Lyophilized |
| Description | FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules. TISSUE SPECIFICITY: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues. Also known as: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65. |
| Specificity | Appears to be specific for SYTI. |
Application Details
| Application Notes | IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Purity | whole serum |
| Storage | Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Restrictions | For Research Use only |
Images
Publications
| Publications |
Sutton, Davletov, Berghuis et al.: "Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/phospholipid-binding fold." in: Cell, Vol. 80, Issue 6, pp. 929-38, 1995 (PubMed).
Schivell, Batchelor, Bajjalieh: "Isoform-specific, calcium-regulated interaction of the synaptic vesicle proteins SV2 and synaptotagmin." in: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27770-5, 1996 (PubMed). Shao, Fernandez, Suedhof et al.: "Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?" in: Biochemistry, Vol. 37, Issue 46, pp. 16106-15, 1998 (PubMed). Coppola, Magnin-Luthi, Perret-Menoud et al.: "Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32756-62, 2001 (PubMed). Fernandez, Arauc, Ubach et al.: "Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine." in: Neuron, Vol. 32, Issue 6, pp. 1057-69, 2001 (PubMed). Heindel, Schmidt, Veit: "Palmitoylation sites and processing of synaptotagmin I, the putative calcium sensor for neurosecretion." in: FEBS letters, Vol. 544, Issue 1-3, pp. 57-62, 2003 (PubMed). Schivell, Mochida, Kensel-Hammes et al.: "SV2A and SV2C contain a unique synaptotagmin-binding site." in: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, 2005 (PubMed). |
Alternatives
Alternatives for antigen "Synapstotagmin I (SYT1)", type "Antibodies"
| Hosts | Rabbit (18), Mouse (4), Goat (1) |
| Reactivities | Human (17), Mouse (Murine) (13), Rat (Rattus) (12), Cow (Bovine) (7), Chicken (4), Dog (Canine) (2), Monkey (2) |
| Applications | Western Blotting (WB) (17), Immunofluorescence (IF) (9), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1), Immunoprecipitation (IP) (1) |
| Epitopes | pSer309 (4), N-Term Synaptotagmin I SytI p65 SVP65 (1), pThr202 (1) |
