Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN350919

Synaptotagmin I (SYT1) (Cytoplasmic Loop), (N-Term) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
P65, SVP65, SYT, AW124717, G630098F17Rik, SytI, CG3139, D. Syt I, DSYT, DSYT2, DSypt, DSyt, Dmel\\CG3139, Droso1, Dsyt2, SYT I, SYT1, Syt, Syt 1, Syt I, Syt-1, dSYT, dsyt1, dsytI, l(2)23AB1, l(2)23Ba, ... show more
Epitope
»Alternatives Cytoplasmic Loop, N-Term
Reactivity
»Alternatives Rat (Rattus)
Host
»Alternatives Sheep
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN350919
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 7 to 8 Business Days
Immunogen A synthetic peptide from the 3d extracellular loop of rat TRPV2 (VRL-1) conjugated to an immunogenic carrier protein was used as the immunogen.
Specificity Specific for TRPV2.
Purity whole serum
Alternative Name Synaptotagmin-1 (Synaptotagmin I, SytI, p65, SVP65)
Background Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2.
Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules.
Tissue specificity: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues. Also known as: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65.
UniProt Q9WUD2
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.
Expiry Date 12 months
Sutton, Davletov, Berghuis et al.: "Structure of the first C2 domain of synaptotagmin I: a novel Ca2+/phospholipid-binding fold." in: Cell, Vol. 80, Issue 6, pp. 929-38, 1995 (PubMed).

Schivell, Batchelor, Bajjalieh: "Isoform-specific, calcium-regulated interaction of the synaptic vesicle proteins SV2 and synaptotagmin." in: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27770-5, 1996 (PubMed).

Shao, Fernandez, Suedhof et al.: "Solution structures of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I: does Ca2+ induce a conformational change?" in: Biochemistry, Vol. 37, Issue 46, pp. 16106-15, 1998 (PubMed).

Coppola, Magnin-Luthi, Perret-Menoud et al.: "Direct interaction of the Rab3 effector RIM with Ca2+ channels, SNAP-25, and synaptotagmin." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 32756-62, 2001 (PubMed).

Fernandez, Arauc, Ubach et al.: "Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine." in: Neuron, Vol. 32, Issue 6, pp. 1057-69, 2001 (PubMed).

Heindel, Schmidt, Veit: "Palmitoylation sites and processing of synaptotagmin I, the putative calcium sensor for neurosecretion." in: FEBS letters, Vol. 544, Issue 1-3, pp. 57-62, 2003 (PubMed).

Schivell, Mochida, Kensel-Hammes et al.: "SV2A and SV2C contain a unique synaptotagmin-binding site." in: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, 2005 (PubMed).

Alternatives for antigen "Synaptotagmin I (SYT1)", type "Antibodies"
Hosts (100), (20), (4), (3), (2), (1)
Reactivities (107), (85), (81), (43), (42), (40), (36), (3), (3), (2), (1), (1)
Applications (88), (32), (31), (30), (22), (21), (14), (8), (3)
Conjugates (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (24), (17), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Beacon