Synaptotagmin V (SYT5) antibody

Antigen: Synaptotagmin V (SYT5)

Synonyms: CG13462, CG3020, CG42333, DmelCG42333, dsytbeta, Syt beta, Syt V, sytbeta, SytV, SytIX, MGC105442, Sytv, syt5, MGC173836, zgc:92682, MGC151577, ARABIDOPSIS THALIANA SYNAPTOTAGMIN HOMOLOG E, ATSYTE, NTMC2T2.1, NTMC2TYPE2.1, synaptotagmin 5, SYNAPTOTAGMIN HOMOLOG E, SYT5, SYTE, T25N20.15

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350928

Additional Information

Alternative name: Synaptotagmin-5 (Synaptotagmin V, SytV, Synaptotagmin IX, Syt5)

UniProt: P47861

Immunogen: A synthetic peptide as a part of rat, mouse Synaptotagmin-5 (Synaptotagmin V, SytV, Synaptotagmin IX, Syt5) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBUNIT: Homodimer. Can also form heterodimers. Interacts with both alpha- and beta-tubulin. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Note: In mast cells, localizes to the endocytic recycling compartment. Also known as: Synaptotagmin 5, Synaptotagmin V, SytV, Synaptotagmin IX, Syt5.

Specificity: Appears to be specific for SYT5.

Synonyms: CG13462, CG3020, CG42333, DmelCG42333, dsytbeta, Syt beta, Syt V, sytbeta, SytV, SytIX, MGC105442, Sytv, syt5, MGC173836, zgc:92682, MGC151577, ARABIDOPSIS THALIANA SYNAPTOTAGMIN HOMOLOG E, ATSYTE, NTMC2T2.1, NTMC2TYPE2.1, synaptotagmin 5, SYNAPTOTAGMIN HOMOLOG E, SYT5, SYTE, T25N20.15

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse Synaptotagmin-5 (Synaptotagmin V, SytV, Synaptotagmin IX, Syt5): whole serum (ABIN350928) at 1: 2000 dilution. DAPI counter stained.

Publications

Hudson, Birnbaum: "Identification of a nonneuronal isoform of synaptotagmin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 13, pp. 5895-9, 1995 (PubMed).

Craxton, Goedert: "Synaptotagmin V: a novel synaptotagmin isoform expressed in rat brain." in: FEBS letters, Vol. 361, Issue 2-3, pp. 196-200, 1995 (PubMed).

Fukuda, Kanno, Mikoshiba: "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31421-7, 1999 (PubMed).

Fukuda, Kowalchyk, Zhang et al.: "Synaptotagmin IX regulates Ca2+-dependent secretion in PC12 cells." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4601-4, 2002 (PubMed).

Haberman, Grimberg, Fukuda et al.: "Synaptotagmin IX, a possible linker between the perinuclear endocytic recycling compartment and the microtubules." in: Journal of cell science, Vol. 116, Issue Pt 21, pp. 4307-18, 2003 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).