Syntaxin 1A (Brain) (STX1A) antibody

Antigen: Syntaxin 1A (Brain) (STX1A)

Synonyms: STX1A, stx1, sx1a, hpc-1, p35-1, MGC145298, DKFZp459F183, STX1, HPC-1, CG10716, CG18615, CG5448, CT30033, Dm Syx1, DmSyx1A, SYX1A, Syt1A, Syx, Syx1, SyxA, anon-EST:Gibbs4, anon-WO02059370.54, dSyx1, l(3)06737, synt, syt-1A, syx-1, syx-1A, DmelCG31136, CG31136

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350936

Additional Information

Alternative name: Syntaxin-1A (Syntaxin 1A, STX1A)

UniProt: P32851

Immunogen: A synthetic peptide as a part of rat, mouse Syntaxin-1A (Syntaxin 1A, STX1A, P35A, Sap) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. SUBUNIT: Interacts with SYTL4, VAPA and SYBU. Found in a complex with VAMP8 andSNAP23. Part of the SNARE core complex containingSNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds STXBP6. Found in a ternary complex with STX1A andSNAP25. SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane protein. TISSUE SPECIFICITY: Expressed predominantly in cerebral cortex, hippocampus, cerebellum, adrenal medulla and retina with weak expression detected in non-neuronal tissues. Also known as: Syntaxin-1A, Synaptotagmin-associated 35 kDa protein, P35A, Neuron-specific antigen HPC-1, Sap, Syntaxin 1A.

Specificity: Appears to be specific for Syntaxin-1A.

Synonyms: STX1A, stx1, sx1a, hpc-1, p35-1, MGC145298, DKFZp459F183, STX1, HPC-1, CG10716, CG18615, CG5448, CT30033, Dm Syx1, DmSyx1A, SYX1A, Syt1A, Syx, Syx1, SyxA, anon-EST:Gibbs4, anon-WO02059370.54, dSyx1, l(3)06737, synt, syt-1A, syx-1, syx-1A, DmelCG31136, CG31136

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Yoshida, Oho, Omori et al.: "HPC-1 is associated with synaptotagmin and omega-conotoxin receptor." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 24925-8, 1993 (PubMed).

Inoue, Obata, Akagawa: "Cloning and sequence analysis of cDNA for a neuronal cell membrane antigen, HPC-1." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10613-9, 1992 (PubMed).

Bennett, Garcuia-Arraruas, Elferink et al.: "The syntaxin family of vesicular transport receptors." in: Cell, Vol. 74, Issue 5, pp. 863-73, 1993 (PubMed).

Sutton, Fasshauer, Jahn et al.: "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." in: Nature, Vol. 395, Issue 6700, pp. 347-53, 1998 (PubMed).

Fasshauer, Antonin, Margittai et al.: "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties." in: The Journal of biological chemistry, Vol. 274, Issue 22, pp. 15440-6, 1999 (PubMed).

Misura, Scheller, Weis: "Three-dimensional structure of the neuronal-Sec1-syntaxin 1a complex." in: Nature, Vol. 404, Issue 6776, pp. 355-62, 2000 (PubMed).

Lerman, Robblee, Fairman et al.: "Structural analysis of the neuronal SNARE protein syntaxin-1A." in: Biochemistry, Vol. 39, Issue 29, pp. 8470-9, 2000 (PubMed).

Scales, Hesser, Masuda et al.: "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28271-9, 2002 (PubMed).

Ernst, Brunger: "High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8630-6, 2003 (PubMed).