Syntaxin-1A, -1B antibody

Details for Product No. ABIN350936, Supplier: Log in to see
Rat (Rattus), Mouse (Murine), Human
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from mouse Syntaxin-1A conjugated to blue carrier protein was used as the antigen.
Specificity Specific for Syntaxin-1A & Syntaxin-1B.
Purification Whole serum
Background Function: Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.
Subunit: Interacts with SYTL4, VAPA and SYBU. Found in a complex with VAMP8 andSNAP23. Part of the SNARE core complex containingSNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds STXBP6. Found in a ternary complex with STX1A andSNAP25.
Subcellular location: Membrane, Single-pass type IV membrane protein.
Tissue specificity: Expressed predominantly in cerebral cortex, hippocampus, cerebellum, adrenal medulla and retina with weak expression detected in non-neuronal tissues. Also known as: Syntaxin-1A, Synaptotagmin-associated 35 kDa protein, P35A, Neuron-specific antigen HPC-1, Sap, Syntaxin 1A.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Ernst, Brunger: "High resolution structure, stability, and synaptotagmin binding of a truncated neuronal SNARE complex." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8630-6, 2003 (PubMed).

Scales, Hesser, Masuda, Scheller: "Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28271-9, 2002 (PubMed).

Lerman, Robblee, Fairman, Hughson: "Structural analysis of the neuronal SNARE protein syntaxin-1A." in: Biochemistry, Vol. 39, Issue 29, pp. 8470-9, 2000 (PubMed).

Misura, Scheller, Weis: "Three-dimensional structure of the neuronal-Sec1-syntaxin 1a complex." in: Nature, Vol. 404, Issue 6776, pp. 355-62, 2000 (PubMed).

Fasshauer, Antonin, Margittai, Pabst, Jahn: "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties." in: The Journal of biological chemistry, Vol. 274, Issue 22, pp. 15440-6, 1999 (PubMed).

Sutton, Fasshauer, Jahn, Brunger: "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." in: Nature, Vol. 395, Issue 6700, pp. 347-53, 1998 (PubMed).

Bennett, García-Arrarás, Elferink, Peterson, Fleming, Hazuka, Scheller: "The syntaxin family of vesicular transport receptors." in: Cell, Vol. 74, Issue 5, pp. 863-73, 1993 (PubMed).

Yoshida, Oho, Omori, Kuwahara, Ito, Takahashi: "HPC-1 is associated with synaptotagmin and omega-conotoxin receptor." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 24925-8, 1993 (PubMed).

Inoue, Obata, Akagawa: "Cloning and sequence analysis of cDNA for a neuronal cell membrane antigen, HPC-1." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10613-9, 1992 (PubMed).