Syntaxin-binding protein 1 (STXB1, Unc-18A) (Internal Region) antibody

Antigen: Syntaxin-binding protein 1 (STXB1, Unc-18A)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350945

Additional Information

UniProt: P61764

Immunogen: A synthetic peptide from the internal part of human Syntaxin-binding protein 1 (STXB1, p67, N-Sec1, Unc-18A, Unc-18-1, rbSec1) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous with the corresponding sequence in rat, mouse, chicken and monkey.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Also known as: STXB1, p67, N-Sec1, Unc-18A, Unc-18-1, rbSec1.

Specificity: Appears to be specific for Syntaxin-binding protein 1.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Gengyo-Ando, Kitayama, Mukaida et al.: "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 21, pp. 6695-702, 1996 (PubMed).

Gotoh, Yokota, Kikuya et al.: "Genomic structure of MUNC18-1 protein, which is involved in docking and fusion of synaptic vesicles in brain." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21642-7, 1998 (PubMed).

Collins, Yu, Coba et al.: "Proteomic analysis of in vivo phosphorylated synaptic proteins." in: The Journal of biological chemistry, Vol. 280, Issue 7, pp. 5972-82, 2005 (PubMed).

Villén, Beausoleil, Gerber et al.: "Large-scale phosphorylation analysis of mouse liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 5, pp. 1488-93, 2007 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).