Taste Receptor, Type 1, Member 3 (TAS1R3) (4th Cytoplasmic Loop) antibody

Antigen: Taste Receptor, Type 1, Member 3 (TAS1R3)

Synonyms: T1R3, Sac, T1r3, TAS1R3, tas1r3

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350964

Additional Information

Alternative name: taste receptor, type 1, member 3 (Tas1r3, TS1R3)

UniProt: Q923K1

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of rat taste receptor, type 1, member 3 (Tas1r3, T1r3, Sac, Tr3, TS1R3) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses. SUBUNIT: Forms homodimers or heterodimers with TAS1R1 and TAS1R2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in circumvallate, foliate and fungiform taste papillae as well as in taste buds on the palate. Also expressed in testis. Not expressed in brain, heart, kidney, liver or spleen. The topographic distribution in various taste papillae is different from those of other T1R members.<||a href="http://senselab.med.yale.edu/senselab/ordb/"><||/a> Also known as: Taste receptor type 1 member 3, sweet taste receptor T1R3, saccharin preference protein, T1r3, Tr3, Tas1r3, TS1R3.

Specificity: Appears to be specific for TAS1R3.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Nelson, Hoon, Chandrashekar et al.: "Mammalian sweet taste receptors." in: Cell, Vol. 106, Issue 3, pp. 381-90, 2001 (PubMed).

Li, Staszewski, Xu et al.: "Human receptors for sweet and umami taste." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 7, pp. 4692-6, 2002 (PubMed).