Taste Receptor, Type 2, Member 12 Pseudogene (TAS2R12) antibody

Antigen: Taste Receptor, Type 2, Member 12 Pseudogene (TAS2R12)

Synonyms: PS10, T2R12, TAS2R26

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350967

Additional Information

Alternative name: taste receptor, type 2, member 12 pseuene (TAS2R12, T2R12, T2R26, TAS2R26)

UniProt: P59531

Immunogen: A synthetic peptide as a part of human taste receptor, type 2, member 12 pseudogene (TAS2R12, T2R12, T2R26, TAS2R26) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. MISCELLANEOUS: Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli. SIMILARITY: Belongs to the G-protein coupled receptor T2R family Note: This protein was previously referred to as T2R26 by authors form Ref.1, and in a first attempt to adopt one commun nomenclature it is considered to be T2R12. It awaits further confirmation. Useful Links <||a href="http://bioportal.weizmann.ac.il/HORDE/links.html"> The Humanolfactory Data Exploratorium: HORDE <||/a> <||a href="http://senselab.med.yale.edu/senselab/ordb/">olfactory Receptor Data Base: ORDB <||/a> <||a href="http://www.enzim.hu/hmmtop1.1/server/submit.html">transmembrane Analysis: TM analysis<||/a> Also known as: Putative Taste receptor type 2 member 12, T2R12, Taste receptor type 2 member 26, T2R26, TAS2R12, TAS2R26.

Specificity: Appears to be specific for TAS2R12.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

Montmayeur, Matsunami: "Receptors for bitter and sweet taste." in: Current opinion in neurobiology, Vol. 12, Issue 4, pp. 366-71, 2002 (PubMed).

Zhang, Hoon, Chandrashekar et al.: "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." in: Cell, Vol. 112, Issue 3, pp. 293-301, 2003 (PubMed).