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Taste Receptor, Type 2, Member 14 (TAS2R14) (AA 200-250) antibody

Details for Product No. ABIN350969, Supplier: Log in to see
  • mGR16
  • mt2r56
  • T2R14
  • T2R16
  • Tas2r7
  • Tas2r14
  • Tas2r16
  • TRB1
  • TRB4
AA 200-250
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 200-250 of human TAS2R14 conjugated to blue carrier protein was used as the antigen.
Specificity Specific for TAS2R14.
Purification Whole serum
Alternative Name TAS2R14 (TAS2R14 Antibody Abstract)
Background This gene product belongs to the family of candidate taste receptors that are members of the G-protein-coupled receptor superfamily. These proteins are specifically expressed in the taste receptor cells of the tongue and palate epithelia. They are organized in the genome in clusters and are genetically linked to loci that influence bitter perception in mice and humans. In functional expression studies, they respond to bitter tastants. This gene maps to the taste receptor gene cluster on chromosome 12p13.
Function: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.
Miscellaneous: Most taste cells may be activated by a limited number of bitter compounds, individual taste cells can discriminate among bitter stimuli. Also known as: Taste receptor type 2 member 14, T2R14, Taste receptor family B member 1, TRB1, TAS2R14, MGC125491, MGC125492.
Research Area Neurology
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Fischer, Gilad, Man, Pääbo: "Evolution of bitter taste receptors in humans and apes." in: Molecular biology and evolution, Vol. 22, Issue 3, pp. 432-6, 2005

Liu, Qian, Gritsenko, Camp, Monroe, Moore, Smith: "Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 6, pp. 2070-80, 2005

Hillier, Fulton, Fulton, Graves, Pepin, Wagner-McPherson, Layman, Maas, Jaeger, Walker, Wylie, Sekhon, Becker, OLaughlin, Schaller, Fewell, Delehaunty, Miner, Nash, Cordes, Du, Sun, Edwards et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003

Zhang, Hoon, Chandrashekar, Mueller, Cook, Wu, Zuker, Ryba: "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." in: Cell, Vol. 112, Issue 3, pp. 293-301, 2003

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002

Montmayeur, Matsunami: "Receptors for bitter and sweet taste." in: Current opinion in neurobiology, Vol. 12, Issue 4, pp. 366-71, 2002

Adler, Hoon, Mueller, Chandrashekar, Ryba, Zuker: "A novel family of mammalian taste receptors." in: Cell, Vol. 100, Issue 6, pp. 693-702, 2000

Chandrashekar, Mueller, Hoon, Adler, Feng, Guo, Zuker, Ryba: "T2Rs function as bitter taste receptors." in: Cell, Vol. 100, Issue 6, pp. 703-11, 2000