Taste Receptor, Type 2, Member 4 (Tas2r108) (Cytoplasmic Domain) antibody

Antigen: Taste Receptor, Type 2, Member 4 (Tas2r108)

Synonyms: T2R4, MGC163311, T2R16, Tas2r4, T2R1, tas2r4, tas2r1l

Binding Site: Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350974

Additional Information

Alternative name: taste receptor, type 2, member 4 (Tas2r4, T2R4)

UniProt: Q9JKT3

Immunogen: A synthetic peptide from a cytoplasmic domain of mouse taste receptor, type 2, member 4 (Tas2r4, T2R4) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Gustducin-coupled receptor for denatonium and N(6)-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. SUBUNIT: Forms heterodimers with TAS1R3. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in 15% taste bud cells in circumvallate and foliate papillae but only in 2% in fungiform papillae.<||a href="http://www.enzim.hu/hmmtop1.1/server/submit.html" /> Also known as: Tas2r4, Tas2r108, Tas2r8, T2R4, Taste receptor type 2 member 8, TA2R4.

Specificity: Appears to be specific for TAS2R4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Adler, Hoon, Mueller et al.: "A novel family of mammalian taste receptors." in: Cell, Vol. 100, Issue 6, pp. 693-702, 2000 (PubMed).

Chandrashekar, Mueller, Hoon et al.: "T2Rs function as bitter taste receptors." in: Cell, Vol. 100, Issue 6, pp. 703-11, 2000 (PubMed).

Zhang, Hoon, Chandrashekar et al.: "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." in: Cell, Vol. 112, Issue 3, pp. 293-301, 2003 (PubMed).

Nelson, Munger, Boughter: "Taste sensitivities to PROP and PTC vary independently in mice." in: Chemical senses, Vol. 28, Issue 8, pp. 695-704, 2003 (PubMed).

Wu, Chen, Rozengurt: "Genomic organization, expression, and function of bitter taste receptors (T2R) in mouse and rat." in: Physiological genomics, Vol. 22, Issue 2, pp. 139-49, 2005 (PubMed).