Taste Receptor, Type 2, Member 40 (TAS2R40) antibody

Antigen: Taste Receptor, Type 2, Member 40 (TAS2R40)

Synonyms: GPR60, T2R40, MGC126844, MGC126846, Tas2r144, RGD1562314

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350978

Additional Information

Alternative name: taste receptor, type 2, member 40 (TAS2R40, T2R40, T2R58, GPR60)

UniProt: P59535

Immunogen: A synthetic peptide as a part of human taste receptor, type 2, member 40 (TAS2R40, T2R40, T2R58, GPR60) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. MISCELLANEOUS: Several bitter taste receptors are expressed in a single taste receptor cell. Useful Links <||a href="http://bioportal.weizmann.ac.il/HORDE/links.html"> The Humanolfactory Data Exploratorium: HORDE <||/a> <||a href="http://senselab.med.yale.edu/senselab/ordb/">olfactory Receptor Data Base: ORDB<||/a> Also known as: Taste receptor type 2 member 40, T2R40, T2R58, G-protein coupled receptor 60, MGC126844, MGC126846.

Specificity: Appears to be specific for TAS2R40.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Chandrashekar, Mueller, Hoon et al.: "T2Rs function as bitter taste receptors." in: Cell, Vol. 100, Issue 6, pp. 703-11, 2000 (PubMed).

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

Montmayeur, Matsunami: "Receptors for bitter and sweet taste." in: Current opinion in neurobiology, Vol. 12, Issue 4, pp. 366-71, 2002 (PubMed).

Bufe, Hofmann, Krautwurst et al.: "The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides." in: Nature genetics, Vol. 32, Issue 3, pp. 397-401, 2002 (PubMed).

Zhang, Hoon, Chandrashekar et al.: "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." in: Cell, Vol. 112, Issue 3, pp. 293-301, 2003 (PubMed).