Taste Receptor, Type 2, Member 50 (TAS2R50) antibody

Antigen: Taste Receptor, Type 2, Member 50 (TAS2R50)

Synonyms: T2R50, T2R51, TAS2R51, MGC138305

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350992

Additional Information

Alternative name: taste receptor, type 2, member 50 (TAS2R50, T2R51)

UniProt: P59544

Immunogen: A synthetic peptide as a part of human taste receptor, type 2, member 50 (TAS2R50, T2R51) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: TAS2R50 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway. FUNCTION: Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the tongue and exclusively in gustducin-positive cells. MISCELLANEOUS: Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli. Also known as: Taste receptor type 2 member 50, T2R50, T2R51, TAS2R50, MGC138305.

Specificity: Appears to be specific for TAS2R50.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Montmayeur, Matsunami: "Receptors for bitter and sweet taste." in: Current opinion in neurobiology, Vol. 12, Issue 4, pp. 366-71, 2002 (PubMed).

Bufe, Hofmann, Krautwurst et al.: "The human TAS2R16 receptor mediates bitter taste in response to beta-glucopyranosides." in: Nature genetics, Vol. 32, Issue 3, pp. 397-401, 2002 (PubMed).

Zhang, Hoon, Chandrashekar et al.: "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." in: Cell, Vol. 112, Issue 3, pp. 293-301, 2003 (PubMed).

Conte, Ebeling, Marcuz et al.: "Identification and characterization of human taste receptor genes belonging to the TAS2R family." in: Cytogenetic and genome research, Vol. 98, Issue 1, pp. 45-53, 2003 (PubMed).

Fischer, Gilad, Man et al.: "Evolution of bitter taste receptors in humans and apes." in: Molecular biology and evolution, Vol. 22, Issue 3, pp. 432-6, 2005 (PubMed).