Telomerase Reverse Transcriptase (TERT) (N-Term) antibody

Antigen: Telomerase Reverse Transcriptase (TERT)

Synonyms: TERT, chTERT, Tert, F5E19.190, F5E19_190, TELOMERASE REVERSE TRANSCRIPTASE, tert, TP2, TRT, EST2, TCS1, hEST2, TR, MGC150124

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351001

Additional Information

Alternative name: Telomerase reverse transcriptase (TERT) (n-terminal)

Swiss-Prot: O14746

Immunogen: A synthetic peptide from N-terminal of human Telomerase reverse transcriptase (HEST2, TP2, TERT, EST2, TCS1, TRT) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). SUBUNIT: Component of the telomerase ribonucleoprotein complex at least composed of TEP1, EST1A, POT1 and a telomerase RNA template component (TER). Interacts with PINX1 and MCRS1. SUBCELLULAR LOCATION: Nucleus. Telomere. DISEASE: Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis. Also known as: Telomerase catalytic subunit, HEST2, Telomerase-associated protein 2, HEST2, TP2, TERT, EST2, TCS1, TRT.

Specificity: Appears to be specific for TERT.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Nakamura, Morin, Chapman et al.: "Telomerase catalytic subunit homologs from fission yeast and human." in: Science (New York, N.Y.), Vol. 277, Issue 5328, pp. 955-9, 1997 (PubMed).

Meyerson, Counter, Eaton et al.: "hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization." in: Cell, Vol. 90, Issue 4, pp. 785-95, 1997 (PubMed).

Wick, Zubov, Hagen: "Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT)." in: Gene, Vol. 232, Issue 1, pp. 97-106, 1999 (PubMed).

Hisatomi, Ohyashiki, Ohyashiki et al.: "Expression profile of a gamma-deletion variant of the human telomerase reverse transcriptase gene." in: Neoplasia (New York, N.Y.), Vol. 5, Issue 3, pp. 193-7, 2003 (PubMed).

Song, Li, Chen et al.: "Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length." in: Biochemical and biophysical research communications, Vol. 316, Issue 4, pp. 1116-23, 2004 (PubMed).

Tao, Wollscheid, OBrien et al.: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005 (PubMed).

Armanios, Chen, Chang et al.: "Haploinsufficiency of telomerase reverse transcriptase leads to anticipation in autosomal dominant dyskeratosis congenita." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 44, pp. 15960-4, 2005 (PubMed).

Nousiainen, Silljue, Sauer et al.: "Phosphoproteome analysis of the human mitotic spindle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 14, pp. 5391-6, 2006 (PubMed).

Matsubara, Murata, Watanabe et al.: "Coronary artery disease and a functional polymorphism of hTERT." in: Biochemical and biophysical research communications, Vol. 348, Issue 2, pp. 669-72, 2006 (PubMed).

Tsakiri, Cronkhite, Kuan et al.: "Adult-onset pulmonary fibrosis caused by mutations in telomerase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 18, pp. 7552-7, 2007 (PubMed).