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Thyroglobulin antibody (TG) (AA 310-360)

Details for Product anti-TG Antibody No. ABIN351005, Supplier: Log in to see
Antigen
  • TG
  • cb717
  • AITD3
  • TGN
  • Tgn
  • cog
Epitope
AA 310-360
42
5
5
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
298
29
22
3
3
1
1
1
1
Host
Sheep
235
57
7
4
1
Clonality
Polyclonal
Conjugate
This Thyroglobulin antibody is un-conjugated
9
9
3
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
134
88
87
61
29
26
14
9
9
6
5
4
3
3
2
2
2
2
2
2
1
1
1
1
Supplier
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Immunogen A synthetic peptide from aa region 310-360 of mouse TGN38 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.
Specificity Specific for TGN38 (or TGN46, TGN48, TGN51 in human).
Purification Whole serum
Alternative Name TGN (TG Antibody Abstract)
Background Function: May be involved in regulating membrane traffic to and from trans-Golgi network.
Subcellular location: Cell membrane, Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane, Single-pass type I membrane protein. Note: Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
Tissue specificity: Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Miscellaneous: Not found in strains BALB/c, C57BL/6 and DBA/2. Also known as: Trans-Golgi network protein TGN51, TGN46, TGN48, TGN38 homolog, Trans-Golgi network integral membrane protein 2, TGOLN2.
Pathways
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Dai, Jin, Sheng et al.: "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." in: Journal of proteome research, Vol. 6, Issue 1, pp. 250-62, 2007 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Wu, MacCoss, Mardones et al.: "Organellar proteomics reveals Golgi arginine dimethylation." in: Molecular biology of the cell, Vol. 15, Issue 6, pp. 2907-19, 2004 (PubMed).

Kasai, Takahashi, Murakami et al.: "Strain-specific presence of two TGN38 isoforms and absence of TGN41 in mouse." in: The Journal of biological chemistry, Vol. 270, Issue 24, pp. 14471-6, 1995 (PubMed).