Transferrin Receptor (p90, CD71) (TFRC) (N-Term) antibody

Antigen: Transferrin Receptor (p90, CD71) (TFRC)

Synonyms: TFR, CD71, TFR1, TRFR, TR, p90, Trfr, Mtvr1, Mtvr-1, AI195355, AI426448, AU015758, 2610028K12Rik, E430033M20Rik, TFRC, tfr, cd71, tfr1, trfr, DKFZp469M0811, LOC100219280

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351035

Additional Information

Alternative name: Transferrin receptor protein 1 (CD71, TfR, TR, p90) (n-terminal region)

Swiss-Prot: P02786

Immunogen: A synthetic peptide from n-terminal region of human Transferrin receptor protein 1 (CD71, TfR, TR, p90) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemia resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.Canine and feline parvoviruses bind human and feline transferrin receptors and use these receptors to enter and infect cells. Subcellular location: Cell membrane; Single-pass type II membrane protein. Melanosome. Also known as: CD71, TfR, TR, p90, T9, Transferrin receptor.

Specificity: Appears to be specific for Transferrin receptor.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Chicz, Urban, Lane et al.: "Predominant naturally processed peptides bound to HLA-DR1 are derived from MHC-related molecules and are heterogeneous in size." in: Nature, Vol. 358, Issue 6389, pp. 764-8, 1992 (PubMed).

Baynes, Shih, Hudson et al.: "Characterization of transferrin receptor released by K562 erythroleukemia cells." in: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), Vol. 197, Issue 4, pp. 416-23, 1991 (PubMed).

Schneider, Owen, Banville et al.: "Primary structure of human transferrin receptor deduced from the mRNA sequence." in: Nature, Vol. 311, Issue 5987, pp. 675-8, 1984 (PubMed).

McClelland, Kuehn, Ruddle: "The human transferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence." in: Cell, Vol. 39, Issue 2 Pt 1, pp. 267-74, 1985 (PubMed).

Douabin-Gicquel, Soriano, Ferran et al.: "Identification of 96 single nucleotide polymorphisms in eight genes involved in iron metabolism: efficiency of bioinformatic extraction compared with a systematic sequencing approach." in: Human genetics, Vol. 109, Issue 4, pp. 393-401, 2001 (PubMed).