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RHOA (AA 100-150) antibody

Details for Product No. ABIN351036, Supplier: Log in to see
Antigen
  • ARH12
  • ARHA
  • RHO12
  • RHOH12
  • Arha
  • Arha1
  • Arha2
  • arha
  • rhoa
  • xrhoa
  • RHO1
  • ras homolog family member A
  • ras homolog gene family, member A
  • RHOA
  • Rhoa
  • rhoa-a
Epitope
AA 100-150
18
15
11
10
4
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
128
60
52
4
3
1
1
1
1
Host
Rabbit
85
39
2
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
5
5
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
100
51
44
23
21
18
14
10
5
4
3
2
1
Options
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Immunogen A synthetic peptide from aa region 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan.
Specificity Specific for RhOA.
Purification Whole serum
Background Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. May be an activator of PLCE1.
Subunit: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with RGNEF.
Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Cytoplasm, cytoskeleton. Also known as: Arha, Arha2, ras homolog gene family member A, MGC72339.
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-RHOA (AA 100-150) antibody (ABIN351036) WB on rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA (Arha...
 image for anti-RHOA (AA 100-150) antibody (ABIN351036) IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Rabbit ant...
 image for anti-RHOA (AA 100-150) antibody (ABIN351036) IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Rabbit ant...
Background publications El-Sibai, Pertz, Pang, Yip, Lorenz, Symons, Condeelis, Hahn, Backer: "RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells." in: Experimental cell research, Vol. 314, Issue 7, pp. 1540-52, 2008 (PubMed).

Dubash, Wennerberg, Garcuia-Mata, Menold, Arthur, Burridge: "A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 3989-98, 2007 (PubMed).

Snyder, Worthylake, Rossman, Betts, Pruitt, Siderovski, Der, Sondek: "Structural basis for the selective activation of Rho GTPases by Dbl exchange factors." in: Nature structural biology, Vol. 9, Issue 6, pp. 468-75, 2002 (PubMed).

Rihet, Vielh, Camonis, Goud, Chevillard, de Gunzburg: "Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors." in: Journal of cancer research and clinical oncology, Vol. 127, Issue 12, pp. 733-8, 2001 (PubMed).

Yoshimura, Sakai, Nakashima, Nozawa, Shinoda, Sakai, Yamada: "Differential expression of Rho family GTP-binding proteins and protein kinase C isozymes during C6 glial cell differentiation." in: Brain research. Molecular brain research, Vol. 45, Issue 1, pp. 90-8, 1997 (PubMed).