Ras Homolog Gene Family, Member A (RHOA) antibody

Antigen: Ras Homolog Gene Family, Member A (RHOA)

Synonyms: ARHA, ARH12, RHO12, RHOH12, Arha, Arha1, Arha2, arha, arha2, xrhoa, MGC53405, arh12, rho12, rhoh12, MGC69542

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351036

Additional Information

Alternative name: Transforming protein RhoA (Arha, Arha2)

UniProt: P61586

Immunogen: A synthetic peptide as a part of human, rat, mouse, chicken, dog, bovine, frog, zebrafish, orangutan Transforming protein RhoA (Arha, Arha2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. May be an activator of PLCE1. SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with RGNEF. SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Also known as: Arha, Arha2, ras homolog gene family member A, MGC72339.

Specificity: Appears to be specific for RhOA.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum (ABIN351036) at 1 : 2000 dilution. One single band is detected. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum (ABIN351036, in red), Sheep antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (ABIN350869, in green), DAPI counterstained appearing in blue.

IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum (ABIN351036, in red), Sheep antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (ABIN350869, in green), DAPI counterstained appearing in blue.

Publications

Yoshimura, Sakai, Nakashima et al.: "Differential expression of Rho family GTP-binding proteins and protein kinase C isozymes during C6 glial cell differentiation." in: Brain research. Molecular brain research, Vol. 45, Issue 1, pp. 90-8, 1997 (PubMed).

Rihet, Vielh, Camonis et al.: "Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors." in: Journal of cancer research and clinical oncology, Vol. 127, Issue 12, pp. 733-8, 2001 (PubMed).

Snyder, Worthylake, Rossman et al.: "Structural basis for the selective activation of Rho GTPases by Dbl exchange factors." in: Nature structural biology, Vol. 9, Issue 6, pp. 468-75, 2002 (PubMed).

Dubash, Wennerberg, Garcuia-Mata et al.: "A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 3989-98, 2007 (PubMed).

El-Sibai, Pertz, Pang et al.: "RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells." in: Experimental cell research, Vol. 314, Issue 7, pp. 1540-52, 2008 (PubMed).