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Ras Homolog Gene Family, Member A (RHOA) (AA 100-150) antibody

Details for Product No. ABIN351038
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Antigen
Synonyms arha, arha2, xrhoa, arh12, rho12, rhoh12, ARH12, ARHA, RHO12, RHOH12, Arha, Arha2, RHO1, rhoa, Arha1, Rhoa, wu:fa66g04, wu:fb26g07, zgc:66058, zgc:85805
Epitope
AA 100-150
(17), (8), (7), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine)
(92), (52), (49), (25), (25), (13), (1), (1), (1), (1)
Host
Sheep
(70), (19), (2), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(73), (38), (22), (20), (17), (8), (2), (2), (1)
Pubmed 5 references available
Quantity 100 µL
Options
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Catalog No. ABIN351038
454.67 $
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Immunogen A synthetic peptide from aa region 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan.
Specificity Specific for RhOA.
Purification Whole serum
Alternative Name RhoA
Background Function: Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. May be an activator of PLCE1.
Subunit: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with RGNEF.
Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Cytoplasm, cytoskeleton. Also known as: Arha, Arha2, ras homolog gene family member A, MGC72339.
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-Ras Homolog Gene Family, Member A (RHOA) (AA 100-150) antibody IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Sheep antibody to Transforming protein RhoA (Arha, Arha2): whole serum (ABIN351038, in red),  Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (ABIN350867, in green), DAPI counterstained.
anti-Ras Homolog Gene Family, Member A (RHOA) (AA 100-150) antibody (2) IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Sheep antibody to Transforming protein RhoA (Arha, Arha2): whole serum (ABIN351038, in red), Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (ABIN350867, in green), DAPI counterstained appearing in blue.
anti-Ras Homolog Gene Family, Member A (RHOA) (AA 100-150) antibody (3) IF (triple labeling, confocal) on rat trigeminal at 1 : 500 dilution using Sheep antibody to Transforming protein RhoA (Arha, Arha2): whole serum (ABIN351038, in red), Rabbit antibody to extracellular, N-terminal part of Sortilin (Neurotensin receptor 3, NTR3, Sort1): whole serum (ABIN350867, in green), DAPI counterstained appearing in blue.
General Yoshimura, Sakai, Nakashima et al.: "Differential expression of Rho family GTP-binding proteins and protein kinase C isozymes during C6 glial cell differentiation." in: Brain research. Molecular brain research, Vol. 45, Issue 1, pp. 90-8, 1997 (PubMed).

Rihet, Vielh, Camonis et al.: "Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors." in: Journal of cancer research and clinical oncology, Vol. 127, Issue 12, pp. 733-8, 2001 (PubMed).

Snyder, Worthylake, Rossman et al.: "Structural basis for the selective activation of Rho GTPases by Dbl exchange factors." in: Nature structural biology, Vol. 9, Issue 6, pp. 468-75, 2002 (PubMed).

Dubash, Wennerberg, Garcuia-Mata et al.: "A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 3989-98, 2007 (PubMed).

El-Sibai, Pertz, Pang et al.: "RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells." in: Experimental cell research, Vol. 314, Issue 7, pp. 1540-52, 2008 (PubMed).

Validation Images
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