rkA (High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A) (Extracellular Domain, N-Term) antibody

Antigen: rkA (High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A)

Binding Site: Extracellular Domain, N-Term

Clonality: Polyclonal

Host: Sheep

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351061

Additional Information

UniProt: P35739

Immunogen: A synthetic peptide from the extracellular domain of rat TrkA (High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF. TISSUE SPECIFICITY: Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues. Also known as: High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor.

Specificity: Appears to be specific for TrkA.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Meakin, Suter, Drinkwater et al.: "The rat trk protooncogene product exhibits properties characteristic of the slow nerve growth factor receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 6, pp. 2374-8, 1992 (PubMed).

Barker, Lomen-Hoerth, Gensch et al.: "Tissue-specific alternative splicing generates two isoforms of the trkA receptor." in: The Journal of biological chemistry, Vol. 268, Issue 20, pp. 15150-7, 1993 (PubMed).

Qian, Riccio, Zhang et al.: "Identification and characterization of novel substrates of Trk receptors in developing neurons." in: Neuron, Vol. 21, Issue 5, pp. 1017-29, 1999 (PubMed).

Kong, Boulter, Weber et al.: "An evolutionarily conserved transmembrane protein that is a novel downstream target of neurotrophin and ephrin receptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 1, pp. 176-85, 2001 (PubMed).

Wooten, Seibenhener, Mamidipudi et al.: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Geetha, Wooten: "Association of the atypical protein kinase C-interacting protein p62/ZIP with nerve growth factor receptor TrkA regulates receptor trafficking and Erk5 signaling." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4730-9, 2003 (PubMed).

Aruevalo, Yano, Teng et al.: "A unique pathway for sustained neurotrophin signaling through an ankyrin-rich membrane-spanning protein." in: The EMBO journal, Vol. 23, Issue 12, pp. 2358-68, 2004 (PubMed).

Chang, Arevalo, Chao: "Ternary complex with Trk, p75, and an ankyrin-rich membrane spanning protein." in: Journal of neuroscience research, Vol. 78, Issue 2, pp. 186-92, 2004 (PubMed).