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TRKA antibody (Neurotrophic Tyrosine Kinase, Receptor, Type 1) (Extracellular Domain)

Details for Product anti-NTRK1 Antibody No. ABIN351062, Supplier: Log in to see
Antigen
  • C80751
  • CAPM1
  • CFTD
  • CTRKA
  • hscp30
  • MTC
  • NEM1
  • OK/SW-cl.5
  • p140-TrkA
  • Tkr
  • TM-5
  • TM3
  • TM5
  • TM30
  • TM30nm
  • TPMsk3
  • trk
  • TRK
  • Trk
  • Trk-A
  • TRK1
  • TRKA
  • TrkA
  • trkA
  • trka
Alternatives
anti-Human TRKA antibody for Immunoprecipitation
Epitope
Extracellular Domain
47
24
23
20
17
16
14
14
13
13
13
13
12
12
11
10
10
9
9
8
6
6
6
6
6
5
5
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3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
380
199
168
2
2
Host
Rabbit
358
50
4
3
2
Clonality
Polyclonal
Conjugate
This TRKA antibody is un-conjugated
16
15
15
10
8
8
8
8
7
7
7
7
7
7
7
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
249
153
89
76
54
46
39
33
19
14
5
4
1
1
Supplier
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Immunogen A synthetic peptide from the extracellular domain of human TrkA conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for TrkA.
Purification Whole serum
Alternative Name TrkA (NTRK1 Antibody Abstract)
Background Function: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1.
Subcellular location: Cell membrane, Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF.
Tissue specificity: Isoform Trka-II is primarily expressed in neuronal cells, isoform Trka-I is found in non-neuronal tissues. Also known as: High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor.
Research Area Cancer
Pathways RTK Signaling, Neurotrophin Signaling Pathway
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006

Fujimoto, Kitazawa, Maeda, Kitazawa: "Methylation adjacent to negatively regulating AP-1 site reactivates TrkA gene expression during cancer progression." in: Oncogene, Vol. 24, Issue 32, pp. 5108-18, 2005

Indo, Mardy, Tsuruta, Karim, Matsuda: "Structure and organization of the human TRKA gene encoding a high affinity receptor for nerve growth factor." in: The Japanese journal of human genetics, Vol. 42, Issue 2, pp. 343-51, 1997

Shelton, Sutherland, Gripp, Camerato, Armanini, Phillips, Carroll, Spencer, Levinson: "Human trks: molecular cloning, tissue distribution, and expression of extracellular domain immunoadhesins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 2, pp. 477-91, 1995

Stephens, Loeb, Copeland, Pawson, Greene, Kaplan: "Trk receptors use redundant signal transduction pathways involving SHC and PLC-gamma 1 to mediate NGF responses." in: Neuron, Vol. 12, Issue 3, pp. 691-705, 1994

Loeb, Stephens, Copeland, Kaplan, Greene: "A Trk nerve growth factor (NGF) receptor point mutation affecting interaction with phospholipase C-gamma 1 abolishes NGF-promoted peripherin induction but not neurite outgrowth." in: The Journal of biological chemistry, Vol. 269, Issue 12, pp. 8901-10, 1994

Hempstead, Martin-Zanca, Kaplan, Parada, Chao: "High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor." in: Nature, Vol. 350, Issue 6320, pp. 678-83, 1991

Klein, Jing, Nanduri, ORourke, Barbacid: "The trk proto-oncogene encodes a receptor for nerve growth factor." in: Cell, Vol. 65, Issue 1, pp. 189-97, 1991

Martin-Zanca, Oskam, Mitra, Copeland, Barbacid: "Molecular and biochemical characterization of the human trk proto-oncogene." in: Molecular and cellular biology, Vol. 9, Issue 1, pp. 24-33, 1989

Martin-Zanca, Hughes, Barbacid: "A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences." in: Nature, Vol. 319, Issue 6056, pp. 743-8, 1986