TrkA,TrkB, TrkC (High affinity nerve growth factor receptor) (pTyr683, pTyr684) antibody

Antigen: TrkA,TrkB, TrkC (High affinity nerve growth factor receptor)

Binding Site: pTyr683, pTyr684

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351067

Additional Information

UniProt: P35739

Immunogen: A synthetic peptide to phospho Y683 & Y684 of rat TrkA (and the corresponding phosphorylated sequences in TrkB and TrkC) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse, human, chicken and zebrafish. Additionally, the antigen shares ~90% identity with the corresponding phosphorylated sequences in TrkB and TrkC.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR. Interacts with NGFR and ARMS. Can form a ternary complex with NGFR and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note: Endocytosed to the endosomes upon treatment of cells with NGF. TISSUE SPECIFICITY: Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues. Also known as: High affinity nerve growth factor receptor, NTRK1, p140-TrkA, Trk-A, Neurotrophic tyrosine kinase receptor type 1, Slow nerve growth factor receptor.

Specificity: Appears to be specific for TrkA, TrkB, TrkC.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. This antibody recognises also the corresponding phosphorylated sequences in TrkB and TrkC.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Meakin, Suter, Drinkwater et al.: "The rat trk protooncogene product exhibits properties characteristic of the slow nerve growth factor receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 6, pp. 2374-8, 1992 (PubMed).

Barker, Lomen-Hoerth, Gensch et al.: "Tissue-specific alternative splicing generates two isoforms of the trkA receptor." in: The Journal of biological chemistry, Vol. 268, Issue 20, pp. 15150-7, 1993 (PubMed).

Qian, Riccio, Zhang et al.: "Identification and characterization of novel substrates of Trk receptors in developing neurons." in: Neuron, Vol. 21, Issue 5, pp. 1017-29, 1999 (PubMed).

Kong, Boulter, Weber et al.: "An evolutionarily conserved transmembrane protein that is a novel downstream target of neurotrophin and ephrin receptors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 1, pp. 176-85, 2001 (PubMed).

Wooten, Seibenhener, Mamidipudi et al.: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Geetha, Wooten: "Association of the atypical protein kinase C-interacting protein p62/ZIP with nerve growth factor receptor TrkA regulates receptor trafficking and Erk5 signaling." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4730-9, 2003 (PubMed).

Aruevalo, Yano, Teng et al.: "A unique pathway for sustained neurotrophin signaling through an ankyrin-rich membrane-spanning protein." in: The EMBO journal, Vol. 23, Issue 12, pp. 2358-68, 2004 (PubMed).

Chang, Arevalo, Chao: "Ternary complex with Trk, p75, and an ankyrin-rich membrane spanning protein." in: Journal of neuroscience research, Vol. 78, Issue 2, pp. 186-92, 2004 (PubMed).