TrkB (BDNF/NT-3 growth factors receptor,TrkB tyrosine kinase, Trk-B) (Extracellular Domain, N-Term) antibody

Antigen: TrkB (BDNF/NT-3 growth factors receptor,TrkB tyrosine kinase, Trk-B)

Binding Site: Extracellular Domain, N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351077

Additional Information

UniProt: Q16620

Immunogen: A synthetic peptide from the extracellular domain of rat, mouse TrkB (BDNF/NT-3 growth factors receptor, TrkB tyrosine kinase, Trk-B) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and ARMS. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Also known as: Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB/GP95-TrkB, Trk-B, BDNF/NT-3 growth factors receptor.

Specificity: Appears to be specific for TrkB.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Haniu, Talvenheimo, Le et al.: "Extracellular domain of neurotrophin receptor trkB: disulfide structure, N-glycosylation sites, and ligand binding." in: Archives of biochemistry and biophysics, Vol. 322, Issue 1, pp. 256-64, 1995 (PubMed).

Nakagawara, Liu, Ikegaki et al.: "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)." in: Genomics, Vol. 25, Issue 2, pp. 538-46, 1995 (PubMed).

Shelton, Sutherland, Gripp et al.: "Human trks: molecular cloning, tissue distribution, and expression of extracellular domain immunoadhesins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 2, pp. 477-91, 1995 (PubMed).

Allen, Dawbarn, Eckford et al.: "Cloning of a non-catalytic form of human trkB and distribution of messenger RNA for trkB in human brain." in: Neuroscience, Vol. 60, Issue 3, pp. 825-34, 1994 (PubMed).

Ultsch, Wiesmann, Simmons et al.: "Crystal structures of the neurotrophin-binding domain of TrkA, TrkB and TrkC." in: Journal of molecular biology, Vol. 290, Issue 1, pp. 149-59, 1999 (PubMed).

Stoilov, Castren, Stamm: "Analysis of the human TrkB gene genomic organization reveals novel TrkB isoforms, unusual gene length, and splicing mechanism." in: Biochemical and biophysical research communications, Vol. 290, Issue 3, pp. 1054-65, 2002 (PubMed).

Greenman, Stephens, Smith et al.: "Patterns of somatic mutation in human cancer genomes." in: Nature, Vol. 446, Issue 7132, pp. 153-8, 2007 (PubMed).