Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1) (1t extracellular loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1)

Synonyms: ANKTM1, CT18073, TRPA, TRPA1, dANKTM1, dTRPA1, Dmel\CG5751, CG5751, Anktm1, trpa1, GLEAN_02449

Epitope: 1t extracellular loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351097

Additional Information

Alternative name: TRPA1 (ANKTM1) (1t extracellular loop)

Swiss-Prot: O75762

Immunogen: A synthetic peptide from the 1t extracellular loop of human TRPA1 (ANKTM1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: The structure of the protein encoded by this gene is highly related to both the protein ankyrin andtransmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. FUNCTION: Receptor-activated non-selective cation channel involved in detection of sensations such as cold, pain or sounds perception. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Also involved in pain sensation triggered by capsaicin, the pungent ingredient in chilli peppers. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). TISSUE SPECIFICITY: Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells. MISCELLANEOUS: Inhibited by ruthenium red, a potent blocker of TRPV channels. Also known as: Transient receptor potential cation channel subfamily A member 1, ankyrin-like withtransmembrane domains protein 1, transformation sensitive protein p120, ANKTM1.

Specificity: Appears to be specific for TRPA1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Jaquemar, Schenker, Trueb: "An ankyrin-like protein with transmembrane domains is specifically lost after oncogenic transformation of human fibroblasts." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7325-33, 1999 (PubMed).

Clapham, Julius, Montell et al.: "International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels." in: Pharmacological reviews, Vol. 57, Issue 4, pp. 427-50, 2005 (PubMed).

Hinman, Chuang, Bautista et al.: "TRP channel activation by reversible covalent modification." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 51, pp. 19564-8, 2006 (PubMed).