Transient Receptor Potential Cation Channel, Subfamily C, Member 1 (TRPC1) (2nd extracellular loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 1 (TRPC1)

Synonyms: TRP1, HTRP-1, MGC133334, MGC133335, Trp1, Mtrp1, Trrp1, TRPC1, trpc1

Epitope: 2nd extracellular loop

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351117

Additional Information

Alternative name: TRPC1 (2d extracellular loop)

Swiss-Prot: Q9QX01

Immunogen: A synthetic peptide from the 2d extracellular loop of human TRPC1 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion. SUBUNIT: Interacts with TRPC3. Interacts with ITPR3. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Seems to be ubiquitous. Also known as: Short transient receptor potential channel 1, TrpC1, TRP-1 protein, Trp1, Trrp1, Trpc1 transient receptor potential cation channel, subfamily C, member 1, Mtrp1, Trp-related protein 1.

Specificity: Appears to be specific for TRPC1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF (confocal) on rat trigeminal at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum (ABIN351117), DAPI counterstained

IF (confocal) on rat brain at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum (ABIN351117), DAPI counterstained appearing in blue.

IF (confocal) on rat brain at 1 : 500 dilution using Rabbit antibody to 2d extracellular loop of TRPC1: whole serum (ABIN351117), DAPI counterstained appearing in blue.

Publications

Publications: Wes, Chevesich, Jeromin et al.: "TRPC1, a human homolog of a Drosophila store-operated channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9652-6, 1995 (PubMed).

Zhu, Chu, Peyton et al.: "Molecular cloning of a widely expressed human homologue for the Drosophila trp gene." in: FEBS letters, Vol. 373, Issue 3, pp. 193-8, 1995 (PubMed).

Zitt, Zobel, Obukhov et al.: "Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion." in: Neuron, Vol. 16, Issue 6, pp. 1189-96, 1996 (PubMed).

Wang, OConnell, Dykeman et al.: "Cloning of Trp1beta isoform from rat brain: immunodetection and localization of the endogenous Trp1 protein." in: The American journal of physiology, Vol. 276, Issue 4 Pt 1, pp. C969-79, 1999 (PubMed).

Lockwich, Liu, Singh et al.: "Assembly of Trp1 in a signaling complex associated with caveolin-scaffolding lipid raft domains." in: The Journal of biological chemistry, Vol. 275, Issue 16, pp. 11934-42, 2000 (PubMed).