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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Transient Receptor Potential Cation Channel, Subfamily C, Member 3, 6, 7 (TRPC3, 6, 7) (1st Cytoplasmic Loop) antibody
1st Cytoplasmic Loop
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|8 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||TRPC3, 6, 7|
|Immunogen||A synthetic peptide from the 1t cytoplasmic loop of human TRPC3 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 85% identity with TRPC6 and TRPC7.|
|Description||FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in brain. Concentrated in cerebellar Purkinje cells and sparsely localized in cerebellar granule lyer, pontine nuclei and thalamus. Lower levels detected in other tissues. Also known as: Short transient receptor potential channel 3, TrpC3, Htrp-3, trp3, transient receptor potential cation channel, subfamily C, member 3.|
|Specificity||Appears to recognise TRPC3, TRPC6 and TRPC7.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Wes, Chevesich, Jeromin et al.: "TRPC1, a human homolog of a Drosophila store-operated channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9652-6, 1995 (PubMed).
Zhu, Jiang, Peyton et al.: "trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." in: Cell, Vol. 85, Issue 5, pp. 661-71, 1996 (PubMed).
Xu, Li, Guggino et al.: "Coassembly of TRP and TRPL produces a distinct store-operated conductance." in: Cell, Vol. 89, Issue 7, pp. 1155-64, 1997 (PubMed).
Zhu, Jiang, Birnbaumer: "Receptor-activated Ca2+ influx via human Trp3 stably expressed in human embryonic kidney (HEK)293 cells. Evidence for a non-capacitative Ca2+ entry." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 133-42, 1998 (PubMed).
Vannier, Zhu, Brown et al.: "The membrane topology of human transient receptor potential 3 as inferred from glycosylation-scanning mutagenesis and epitope immunocytochemistry." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8675-9, 1998 (PubMed).
Kiselyov, Xu, Mozhayeva et al.: "Functional interaction between InsP3 receptors and store-operated Htrp3 channels." in: Nature, Vol. 396, Issue 6710, pp. 478-82, 1998 (PubMed).
Hofmann, Obukhov, Schaefer et al.: "Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol." in: Nature, Vol. 397, Issue 6716, pp. 259-63, 1999 (PubMed).
Boulay, Brown, Qin et al.: "Modulation of Ca(2+) entry by polypeptides of the inositol 1,4, 5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP): evidence for roles of TRP and IP3R in store depletion-activated Ca(2+) entry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 26, pp. 14955-60, 2000 (PubMed).