Transient Receptor Potential Cation Channel, Subfamily C, Member 3, 6, 7 (TRPC3, 6, 7) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 3, 6, 7 (TRPC3, 6, 7)

Synonyms: Trrp3, TRPC3

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351132

Additional Information

Alternative name: TRPC3, 6, 7 (4th cytoplasmic loop)

Swiss-Prot: Q9JMI9

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of rat TRPC3 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Short transient receptor potential channel 3, transient receptor potential cation channel, subfamily C, member 3.

Specificity: Appears to recognise TRPC3, TRPC6 and TRPC7.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Ohki, Miyoshi, Murata et al.: "A calcium-activated cation current by an alternatively spliced form of Trp3 in the heart." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39055-60, 2001 (PubMed).

Santilluan, Baldi, Katz et al.: "Evidence that TRPC3 is a molecular component of the 1alpha,25(OH)2D3-activated capacitative calcium entry (CCE) in muscle and osteoblast cells." in: The Journal of steroid biochemistry and molecular biology, Vol. 89-90, Issue 1-5, pp. 291-5, 2004 (PubMed).

Wu, Zagranichnaya, Gurda et al.: "A TRPC1/TRPC3-mediated increase in store-operated calcium entry is required for differentiation of H19-7 hippocampal neuronal cells." in: The Journal of biological chemistry, Vol. 279, Issue 42, pp. 43392-402, 2004 (PubMed).

Thebault, Zholos, Enfissi et al.: "Receptor-operated Ca2+ entry mediated by TRPC3/TRPC6 proteins in rat prostate smooth muscle (PS1) cell line." in: Journal of cellular physiology, Vol. 204, Issue 1, pp. 320-8, 2005 (PubMed).

Yildirim, Kawasaki, Birnbaumer: "Molecular cloning of TRPC3a, an N-terminally extended, store-operated variant of the human C3 transient receptor potential channel." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 9, pp. 3307-11, 2005 (PubMed).

Bush, Hood, Papst et al.: "Canonical transient receptor potential channels promote cardiomyocyte hypertrophy through activation of calcineurin signaling." in: The Journal of biological chemistry, Vol. 281, Issue 44, pp. 33487-96, 2006 (PubMed).