Transient Receptor Potential Cation Channel, Subfamily C, Member 3, 6, 7 (TRPC3, 6, 7) (Cytoplasmic Loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 3, 6, 7 (TRPC3, 6, 7)

Synonyms: Trrp3, TRPC3

Binding Site: Cytoplasmic Loop

Clonality: Polyclonal

Host: Sheep

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351135

Additional Information

Alternative name: TRPC3, 6, 7

UniProt: Q9QZC1

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of mouse TRPC3 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in brain. Concentrated in cerebellar Purkinje cells and sparsely localized in cerebellar granule lyer, pontine nuclei and thalamus. Lower levels detected in other tissues. Also known as: Short transient receptor potential channel 3, transient receptor potential cation channel, subfamily C, member 3, TrpC3, Transient receptor protein 3, Mtrp3, Receptor-activated cation channel TRP3, Trp-related protein 3, Trrp3, MGC124333.

Specificity: Appears to recognise TRPC3, TRPC6 and TRPC7.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Zhu, Jiang, Peyton et al.: "trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." in: Cell, Vol. 85, Issue 5, pp. 661-71, 1996 (PubMed).

Mori, Takada, Okada et al.: "Differential distribution of TRP Ca2+ channel isoforms in mouse brain." in: Neuroreport, Vol. 9, Issue 3, pp. 507-15, 1998 (PubMed).

Ohki, Miyoshi, Murata et al.: "A calcium-activated cation current by an alternatively spliced form of Trp3 in the heart." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39055-60, 2001 (PubMed).

Ramsey, Delling, Clapham: "An introduction to TRP channels." in: Annual review of physiology, Vol. 68, pp. 619-47, 2006 (PubMed).

Lee, Cherednichenko, Pessah et al.: "Functional coupling between TRPC3 and RyR1 regulates the expressions of key triadic proteins." in: The Journal of biological chemistry, Vol. 281, Issue 15, pp. 10042-8, 2006 (PubMed).

Rodruiguez-Santiago, Mendoza-Torres, Jimuenez-Bremont et al.: "Knockout of the trcp3 gene causes a recessive neuromotor disease in mice." in: Biochemical and biophysical research communications, Vol. 360, Issue 4, pp. 874-9, 2007 (PubMed).