Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 4 (TRPC4)

Synonyms: TRP4, HTRP4, MGC119570, MGC119571, MGC119572, MGC119573, CCE1, Trp4, Trrp4, STRPC4, TRPC4, TRPC5, Trpc4

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351143

Additional Information

Alternative name: TRPC4 (TRP4) (4th cytoplasmic loop)

Swiss-Prot: Q9QUQ5

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of mouse TRPC4 (TRP4) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Thought to form non-selective a receptor-activated calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. SUBUNIT: Isoform alpha but not isoform beta associates with inositol-1,4,5-triphosphate receptor (ITPR). Interacts with NHERF. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. - TISSUE SPECIFICITY: Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer ofolfactory bulb). Lower levels are detected in other tissues. - TISSUE SPECIFICITY: Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain. Also known as: Short transient receptor potential channel 4, TrpC4, Trp4, Capacitative calcium entry channel 1, CCE1, Trrp4, Receptor-activated cation channel TRP4, Trp-related protein 4, hTrp-4, hTrp4.

Specificity: Appears to be specific for TRPC4.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Petersen, Berridge, Borgese et al.: "Putative capacitative calcium entry channels: expression of Drosophila trp and evidence for the existence of vertebrate homologues." in: The Biochemical journal, Vol. 311 ( Pt 1), pp. 41-4, 1995 (PubMed).

Mori, Takada, Okada et al.: "Differential distribution of TRP Ca2+ channel isoforms in mouse brain." in: Neuroreport, Vol. 9, Issue 3, pp. 507-15, 1998 (PubMed).

Tang, Tang, Chen et al.: "Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 37559-64, 2000 (PubMed).

Freichel, Suh, Pfeifer et al.: "Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice." in: Nature cell biology, Vol. 3, Issue 2, pp. 121-7, 2001 (PubMed).